Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.5K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation, characterization and immune enhancement study of Hericium erinaceus polysaccharide-loaded polyethyleneimine-modified poly(lactic-co-glycolic acid) nanoparticles.

Journal of the science of food and agriculture·2026
Same author

Hematopoiesis could be improved by correcting aberrant bone marrow macrophages polarization in aplastic anemia patients.

Science China. Life sciences·2026
Same author

TGF-β1-triggered maladaptive bone marrow endothelium impedes hematopoietic recovery.

Signal transduction and targeted therapy·2025
Same author

TGF-β inhibition restores hematopoiesis and immune balance via bone marrow EPCs in aplastic anemia.

Experimental & molecular medicine·2025
Same author

Spatially Resolved Electrochemiluminescent Biosensor Combined with Nanopore Screening Devices for Simultaneous Determination of Deoxynivalenol and Aflatoxin B<sub>1</sub>.

Analytical chemistry·2025
Same author

The influencing mechanism of thermo-oxidative aging of waste cotton textiles on mechanical properties of their regenerated fibers.

International journal of biological macromolecules·2025

Related Experiment Video

Updated: Feb 19, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K

Structure-adaptive dual-function poly-T sequences enable a homogeneous label-free colorimetric sensing platform.

Shao-Yu Sun1, Yi Song1, An-Qi Xiao2

  • 1School of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, China. zhouqy@cqust.edu.cn.

The Analyst
|February 17, 2026
PubMed
Summary

Poly-thymine (poly-T) DNA sequences show peroxidase-like activity, enabling a new colorimetric sensor. This sensor detects melamine by observing changes in the DNA

More Related Videos

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.2K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.6K

Related Experiment Videos

Last Updated: Feb 19, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.2K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Research on G-rich nucleic acids is extensive, but the catalytic properties of T-rich sequences remain underexplored.
  • Poly-thymine (poly-T) sequences are typically viewed for their structural roles, not catalytic functions.

Purpose of the Study:

  • To investigate the peroxidase-like activity of poly-T sequences.
  • To develop a novel homogeneous colorimetric sensing platform for melamine detection using poly-T sequences.

Main Methods:

  • Investigated the peroxidase-like activity of poly-T sequences with acetic acid as an activator.
  • Constructed a homogeneous colorimetric sensing platform utilizing long-chain poly-T sequences to catalyze the TMB-H2O2 reaction.
  • Utilized the formation of T-melamine-T structures to induce conformational changes and suppress catalytic activity.

Main Results:

  • Poly-T sequences exhibit peroxidase-like activity, with efficiency increasing with sequence length.
  • The developed sensing platform successfully detects melamine.
  • Melamine binding to poly-T sequences leads to structural changes that significantly inhibit the catalytic activity.

Conclusions:

  • Poly-T sequences possess inherent catalytic properties, acting as activatable peroxidase mimics.
  • A novel, dual-function ssDNA sensing platform integrates catalytic and recognition capabilities for melamine detection.
  • The structure-dependent activity modulation of poly-T sequences allows for rapid, on-site detection via a colorimetric assay.