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Catalytic Wiring of Enzymatic Cascades Using ROS-Flux-Regulated Biodegradable Borophene.

Pranay Saha1, Shraddha Krishnakumar2, Aysenur Yardim1,3

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Summary

Borophene, a 2D boron material, acts as an electrocatalyst in enzyme cascades. Its reactivity with hydrogen peroxide (H2O2) enables dual output biosensors, but reactive oxygen species can limit its use.

Keywords:
boropheneelectrochemical biosensingenzymatic catalysisglucose oxidase‐horseradish peroxidase (GOx‐HRP) cascadereactive oxygen species (ROS)

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Area of Science:

  • Materials Science
  • Electrochemistry
  • Biotechnology

Background:

  • Two-dimensional (2D) borophene, a single-atom-thick boron allotrope, possesses unique conductivity and reactivity.
  • Its electrochemical and enzymatic properties, especially under biological redox conditions, remain largely unexplored.

Purpose of the Study:

  • To investigate the enzymatic and electrochemical behavior of borophene.
  • To explore borophene's potential as an electrocatalyst and transducer in biosensing applications.
  • To understand the impact of reactive oxygen species (ROS) on borophene's performance.

Main Methods:

  • Spectroscopic and microscopic analyses to study borophene degradation.
  • Curcumin-rosocyanine assay for boronic acid detection.
  • Enzymatic cascades using glucose oxidase and horseradish peroxidase.
  • Electroanalytical techniques including cyclic voltammetry, chronoamperometry, and differential pulse voltammetry.

Main Results:

  • Borophene degrades in a concentration-dependent manner when exposed to hydrogen peroxide (H2O2), forming boronic acids.
  • Borophene functions as an electrocatalyst and transducer, enabling dual colorimetric and electrochemical outputs in enzymatic cascades.
  • Efficient wiring of peroxidase reactions was observed via borophene-HRP interfaces, enhancing H2O2 detection.
  • Glucose sensing showed reduced currents due to passivation by reactive oxygen species.

Conclusions:

  • Borophene serves as a versatile platform for catalytic wiring of enzyme cascades.
  • The dynamic regulation of signal output by ROS flux allows for transient, self-reporting biosensors.
  • Stabilization strategies are needed for integrating borophene into long-term bioelectronic devices.