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Unveiling P450 Gene Diversity: Structural Variants and Expression Divergence in Three Aphis gossypii Haplotypes
Weili Jiang1, Muhammad Farhan2, Ruiqi Guo2
1Basic Experimental Teaching Center of Life Sciences, Yangzhou university, Yangzhou, Jiangsu, China.
Archives of Insect Biochemistry and Physiology
|February 17, 2026
Summary
This study identified 58 Cytochrome P450 genes in the cotton-melon aphid, Aphis gossypii, revealing variations in gene structure and expression across different haplotypes. These findings are crucial for understanding pesticide resistance and aphid population dynamics.
Area of Science:
- * Entomology
- * Molecular Biology
- * Genomics
Background:
- * The cotton-melon aphid (Aphis gossypii) is a significant agricultural pest due to its widespread nature and resistance to insecticides.
- * Cytochrome P450 monooxygenases (P450s) play a key role in detoxifying insecticides and plant compounds, contributing to aphid resilience.
- * High-quality genome assemblies for A. gossypii haplotypes (Hap1, Hap3, Hap4) are now available, enabling detailed genetic analysis.
Purpose of the Study:
- * To identify and classify all P450 genes in A. gossypii.
- * To investigate the distribution, structure, and expression patterns of P450 genes across different A. gossypii haplotypes.
- * To explore the relationship between P450 gene variations and insecticide resistance in A. gossypii.
Main Methods:
- * Genome-wide identification and classification of P450 genes into four CYP clans (CYP2, CYP3, CYP4, and mitochondrial).
- * Chromosomal mapping to determine the distribution of P450 genes, with a focus on clustered regions.
- * Comparative analysis of P450 gene structures and expression levels among A. gossypii Hap1, Hap3, and Hap4 haplotypes.
Main Results:
- * A total of 58 P450 genes were identified in A. gossypii, with significant numbers in the CYP3 and CYP4 clans.
- * Over half of the P450 genes are located on chromosome A1, often arranged in clusters.
- * Conserved gene structures were observed for insecticide resistance-related P450 genes across haplotypes, but expression levels varied significantly.
- * Five structural variants of P450 genes were identified, with intron size variation in P450 4C1 genes notably affecting gene expression.
Conclusions:
- * The identified P450 gene repertoire provides a foundation for understanding insecticide metabolism in A. gossypii.
- * Variations in P450 gene structure and expression among haplotypes contribute to differential pesticide resistance.
- * These findings offer valuable insights into aphid population genetics and the evolution of pesticide resistance, aiding in pest management strategies.
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