Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

3.1K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
3.1K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.7K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.7K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.7K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ion chromatography high-resolution mass spectrometry-quality assurance by co-injection of internal standards.

Analytical and bioanalytical chemistry·2026
Same author

Not One Method to Rule Them All: A Comparative Study of Chromatographic Platforms (RP-LC-, HILIC-, SFC-, and IC-HRMS) for Water Analysis.

Analytical chemistry·2025
Same author

Nontarget and Suspect Screening of Fluorinated Ionic Liquids and PFAS in European Wastewaters Using Supercritical Fluid Chromatography.

Environmental science & technology·2025
Same author

Quantification Challenges in Polymer Analysis in Urban Runoff and Wastewater using Pressurized Liquid Extraction and Double-Shot Pyrolysis-Gas Chromatography-Mass Spectrometry.

Analytical chemistry·2025
Same author

Overcoming Matrix Effects in Non-Target Screening: A Novel Strategy for Urban Runoff Analysis.

Analytical chemistry·2025
Same author

Mapping Emerging Contaminants in Wastewater Effluents through Multichromatographic Platform Analysis and Source Correlations.

Environmental science & technology·2025
Same journal

Correction to "Zinc/Silver-Based Hydrogen Sulfide Trapping as an Alternative to Cadmium for Sulfur Stable Isotope Analysis".

Rapid communications in mass spectrometry : RCM·2026
Same journal

The Possibility of X-Type Ions With Linear Saturated Non-Reducing Termini Dissociated From Potasiated Saikosaponins.

Rapid communications in mass spectrometry : RCM·2026
Same journal

Effect of Humidity on Ionisation and Ion Chemistry in Active-Capillary Dielectric Barrier Discharge Ionisation.

Rapid communications in mass spectrometry : RCM·2026
Same journal

Multimodal Transformer Fusion of PTR-ToF-MS Volatiles and Targeted Non-Volatile Metabolites Enables Data-Driven Grading of Baimudan White Tea.

Rapid communications in mass spectrometry : RCM·2026
Same journal

Determination of Residual Nitrilase in Brivaracetam Using Characteristic Peptides by Liquid Chromatography-Mass Spectrometry.

Rapid communications in mass spectrometry : RCM·2026
Same journal

Bioanalysis of Ametryn by UHPLC-MS<sup>3</sup> Coupled With Multiple Fragmentation to Decrease Interference and Enhance Sensitivity.

Rapid communications in mass spectrometry : RCM·2026
See all related articles

Related Experiment Video

Updated: Feb 19, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.5K

Automated Near Real-Time QC for LC-HRMS.

Michael J Mohr1,2, Linus Strähle1, Tobias Bader1

  • 1Zweckverband Landeswasserversorgung, Stuttgart, Germany.

Rapid Communications in Mass Spectrometry : RCM
|February 17, 2026
PubMed
Summary
This summary is machine-generated.

Automated quality control (QC) for liquid chromatography-high-resolution mass spectrometry (LC-HRMS) detects issues in near real-time, preventing sample loss and reruns. This workflow enhances analytical measurement reliability through immediate alerts and trend analysis.

Keywords:
QA/QCautomated QA/QCmultivariate statistical process controlnear real‐time analysisonline LC‐HRMS

More Related Videos

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.7K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.4K

Related Experiment Videos

Last Updated: Feb 19, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.5K
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.7K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.4K

Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Data Science

Background:

  • Traditional quality control (QC) for analytical measurements occurs post-batch, risking sample loss and reruns.
  • Automated, real-time QC during LC-HRMS acquisition can identify issues promptly.
  • This study evaluates a novel QC approach using historical river-water surveillance data.

Purpose of the Study:

  • To develop and evaluate an automated, near real-time quality control workflow for LC-HRMS.
  • To enable immediate detection and documentation of performance excursions during data acquisition.
  • To minimize sample loss and prevent costly reruns through proactive issue flagging.

Main Methods:

  • A modular MATLAB workflow was developed to track isotopically labelled internal standards.
  • Multivariate statistical process control (MSPC) using PCA and Hotelling's T² was applied to peak height, retention time, and mass error.
  • Immediate email alerts were issued, and outcomes were logged to a PostgreSQL database/Grafana dashboard.

Main Results:

  • The workflow successfully flagged issues during simulated instrument malfunctions (power supply failure, pump malfunction).
  • MSPC detected mass error anomalies even when univariate limits were not breached.
  • High agreement was observed between MSPC and univariate thresholds, with minimal Type I and Type II errors.

Conclusions:

  • The developed workflow enables immediate detection, triage, and documentation of performance excursions in LC-HRMS.
  • It supports proactive maintenance and minimizes downtime, safeguarding valuable samples.
  • The workflow is instrument-agnostic and broadly applicable to internal-standardized LC-HRMS methods.