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Updated: Feb 19, 2026

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
Published on: April 17, 2018
Identification and Quantitative Analysis of Unusual Unsaturated Fatty Acid Isomers in Fresh and Dry Marine
Hao Yang1, Anyu Liu1,2, Xinnan Luo1
1School of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.
Abstract:
Edible marine invertebrates are good sources of omega-3 polyunsaturated fatty acids (PUFA). However, these organisms display complex FA profiles with monounsaturated FA featuring unusual double-bond positions and PUFA containing polymethylene-interrupted configurations. Identifying and quantifying these isomers pose major analytical challenges, particularly when coelution occurs. Covalent adduct chemical ionization mass spectrometry enables de novo identification of unusual PUFA and sensitive quantification of trace and coeluted FA due to its high specificity. In this study, we developed a straightforward method for quantifying coeluted polymethylene-interrupted PUFA without standards or specialized columns and analyzed the FA compositions of three marine invertebrates commonly marketed fresh and dried. A total of 100 FA were identified, including several novel odd-chain monounsaturated FA. Marked interspecies differences were observed for various FA, while drying had a small impact. This work provides new insights into the nutritional values of dry marine invertebrates and introduces a practical approach for PUFA isomer analysis.
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