Downregulation of GNPNAT1 impaired proliferation and correlates with immune infiltration in lung adenocarcinoma

Glucosamine‑phosphate N‑acetyltransferase 1(GNPNAT1) is involved in metabolism in eukaryotic cells.Researches have reported that GNPNAT1 promoted the malignant behaviors of cancers.The role of GNPNAT1 in lung adenocarcinoma(LUAD) is little known.We analyzed the LUAD prognosis-associated genes using data from.The Cancer Genome Atlas(TCGA).And GNPNAT1 was identified.which was validated by quantitative real-time polymerase chain reaction, colony formation assay and MTT assay. UALCAN, The Human Protein Atlas(HPA), the Cancer Cell Line Encyclopedia(CCLE), TIMER, and TISIDB were used to analyze its relationship with clinical characteristics, co-expressed genes, immune infiltration, correlation with immunomodulators.GNPNAT1-associated gene, CD40LG was used to build a risk model to evaluate immunotherapy outcomes of patients with LUAD.GNPNAT1 was upregulated in LUAD both at mRNA and protein level in tumor tissues. Based on CD40LG, a risk model could predict the prognosis and immunotherapy outcome of LUAD.GNPNAT1 was an independent prognostic factor for LUAD.Our results have identified a prognosis-related gene that is useful to evaluate immunotherapy outcomes of LUAD patients.The signature may have crucial clinical significance in predicting survival prognosis, and immune infiltration based on GNPNAT1 gene.