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Casting the Caspase Activity and Apoptosis Inhibitor 1 Phosphoregulation Through Global Phosphoproteomes

Pathiyil Sajini Sekhar1, Amal Fahma1, Athira Perunally Gopalakrishnan1

  • 1Centre for Integrative Omics Data Science (CIODS), Yenepoya (Deemed to be University), Mangalore, India.

Omics : a Journal of Integrative Biology
|February 17, 2026
PubMed

Insights

Caspase Activity and Apoptosis Inhibitor 1 (CAAP1) phosphorylation, particularly at sites S203, S89, S312, and T90, is linked to splicing and apoptosis. This CAAP1 phosphoregulatory network offers potential therapeutic targets for cancer.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Proteomics

Background:

  • Caspase Activity and Apoptosis Inhibitor 1 (CAAP1) is a protein that inhibits apoptosis and promotes cell survival.
  • CAAP1's role in cancer progression, migration, and angiogenesis is recognized, but its post-transcriptional regulation, specifically phosphorylation, is not well understood.
  • CAAP1 is an underexplored therapeutic target for cancer treatment.

Purpose of the Study:

  • To investigate the phosphorylation of CAAP1 and its functional significance.
  • To identify key phosphorylation sites (PS) on CAAP1 using global phosphoproteomic data.
  • To explore the biological roles of CAAP1 through co-regulated phosphosites.

Main Methods:

  • Systematic analysis of 885 human phosphoproteomic profiling datasets.
  • Analysis of 172 differential expression datasets.
  • Identification of Class 1 phosphorylation sites (PS) in CAAP1.
  • Examination of co-regulated protein phosphosites and enrichment analysis.

Main Results:

  • Four predominant CAAP1 phosphorylation sites were identified: S203, S89, S312, and T90.
  • Co-regulated phosphosites associated with CAAP1's predominant sites were enriched in splicing-related processes.
  • Strong associations were found between these phosphosites, spliceosome-related proteins, and apoptotic regulators.

Conclusions:

  • The CAAP1 phosphoregulatory network is implicated in splicing mechanisms, apoptosis regulation, and cancer progression.
  • CAAP1 phosphorylation occurs in cellular processes frequently dysregulated in cancer.
  • This study provides a basis for developing therapeutic strategies targeting CAAP1 phosphoregulation in cancer.
Keywords:
apoptosiscancerdata integrationphosphoproteomicssplicing

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