Hydroxytyrosol-loaded zein nanoparticles: A green formulation for pharma/nutraceutical applications

Area of Science:

• Food Science and Technology
• Nanotechnology
• Nutraceuticals

Background:

• Hydroxytyrosol, a potent antioxidant, has poor bioavailability.
• Developing strategies to enhance hydroxytyrosol stability and absorption is crucial for functional foods.

Purpose of the Study:

• To develop a method for preserving hydroxytyrosol's bioactivity.
• To encapsulate hydroxytyrosol within zein nanoparticles for improved delivery.

Main Methods:

• Encapsulation of hydroxytyrosol in zein nanoparticles.
• Characterization of nanoparticle size, zeta potential, and entrapment efficiency.
• Assessment of formulation stability under pasteurization and ionic stress.
• In vitro simulated gastrointestinal digestion and antioxidant activity assays (ORAC).

Main Results:

• Zein nanoparticles achieved ~100 nm diameter with high entrapment efficiency.
• Formulations were stable after pasteurization and up to 100 mM NaCl.
• Nanocarriers protected hydroxytyrosol from degradation during simulated digestion.
• Hydroxytyrosol's antioxidant activity was maintained, confirmed by ORAC assay.

Conclusions:

• Hydroxytyrosol-loaded zein nanoparticles offer a promising platform for enhancing its use in functional foods.
• This strategy improves hydroxytyrosol stability, bioavailability, and bioactivity for nutraceutical applications.