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Annual Banned-Substance Review 18th Edition-Analytical Approaches in Human Sports Drug Testing 2024/2025
Mario Thevis1,2, Tiia Kuuranne3, Hans Geyer1,2
1Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, am Sportpark Müngersdorf 6, Cologne/Bonn, Germany.
This review covers anti-doping research from October 2024 to September 2025, focusing on new drug entities and analytical methods for sports drug testing. It highlights anabolic agents and emerging gene doping practices relevant to the World Anti-Doping Agency
Area of Science:
- Analytical Chemistry
- Biochemistry
- Sports Science
Background:
- Anti-doping research is expanding alongside drug development.
- Accurate analytical methods are crucial for routine doping control.
- This review covers literature published from October 2024 to September 2025.
Purpose of the Study:
- To review analytical approaches in human sports drug testing.
- To discuss established and new doping agents in the context of the 2025 World Anti-Doping Agency Prohibited List.
- To highlight advancements in detecting anabolic agents and gene doping practices.
Main Methods:
- Literature review of scientific publications.
- Analysis of data on drug metabolism, elimination, and detection.
- Focus on anabolic agents (steroids, SARMs, protein therapeutics) and gene doping (oligonucleotides, transgenes, gene editing).
Main Results:
- Investigated metabolic fate and detection of anabolic agents.
- Presented detection strategies for new drug candidates.
- Observed continued expansion in testing for gene doping practices.
Conclusions:
- The review provides comprehensive information on analytical challenges in sports drug testing.
- New anabolic substances and gene doping methods require ongoing analytical method development.
- These findings are relevant for current and future sports drug testing programs.
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