Related Experiment Video
Updated: Feb 19, 2026
A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
Published on: April 16, 2018
Impact of Endogenous Melatonin Status on Perioperative Oxidative Stress in Pediatric Cardiac Surgery With
Fidan Ismayilova1, Noa Judith Freudenthal2, Nicole Müller2
1Neonatology and Pediatric Intensive Care, Children's Hospital, University of Bonn, Germany.
Objectives:
This study aimed to investigate whether prebypass endogenous melatonin concentrations are associated with perioperative oxidative DNA injury, indexed by urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), and to explore their relationship with postoperative N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels in children undergoing cardiac surgery with cardiopulmonary bypass (CPB).
Design:
A prospective, observational study.
Setting:
A single-center study conducted in a tertiary care hospital.
Participants:
Eighty pediatric patients (neonates to 18 years) undergoing corrective cardiac surgery with CPB.
Interventions:
No experimental intervention was performed; blood and urine samples were collected perioperatively for biochemical analysis.
Measurements And Main Results:
Serum prebypass and postbypass melatonin levels were measured by enzyme-linked immunosorbent assay; urinary 8-OHdG and NT-proBNP were quantified immediately and 24 hours postoperatively. Postbypass melatonin concentrations correlated positively with age (r = 0.288, p = 0.010) and negatively with surgical duration (r = -0.230, p = 0.040). Higher prebypass melatonin levels tended to be associated with lower postoperative urinary 8-OHdG (r = -0.300, p = 0.007). Postoperative 8-OHdG showed strong linear correlations with NT-proBNP both immediately and 24 hours after surgery (r = 0.516, p < 0.001; r = 0.451, p < 0.001). In multivariable analysis, 8-OHdG remained an independent predictor of NT-proBNP, whereas melatonin did not.
Conclusions:
Oxidative DNA injury is strongly associated with postoperative myocardial stress in pediatric patients undergoing CPB. Prebypass melatonin status showed an exploratory, nonindependent relationship with perioperative oxidative stress, while oxidative stress markers were robustly linked to NT-proBNP. These findings delineate a redox-cardiac stress axis in pediatric CPB and support further investigation into the role of endogenous antioxidant capacity in perioperative cardiac stress.
More Related Videos
12:02Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
Published on: July 30, 2016
07:51Standardized Hemorrhagic Shock Induction Guided by Cerebral Oximetry and Extended Hemodynamic Monitoring in Pigs
Published on: May 21, 2019
Related Concept Videos
01:28Cardiomyopathy VII: Pre and Post Operative Nursing Management
01:15Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...