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RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
Published on: September 28, 2011
Prevalence and genotyping of Pseudomonas aeruginosa from food and human sources
Walid S Mousa1, Eman E Abdeen2, Hanem F El-Gendy3
1Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32958, Egypt.
Abstract:
Pseudomonas aeruginosa is a common pathogen of a wide range of nosocomial infections in humans, as well as foodborne illnesses. The current study focuses on the molecular genotyping of P. aeruginosa recovered from human, water, and food specimens. A total of 350 samples, fifty samples each from human, tap water, fish swamp, chicken meat, minced meat, raw milk, and hospital surface from the Menoufiya governorate, Egypt. P. aeruginosa was detected in 14.28%, including human (26%), tap water (18%), fish swamp (18%), chicken meat (12%), minced meat (10%), raw milk (16%), and hospital surface (0%). The results of testing of 50 P. aeruginosa isolates against sixteen antibiotics revealed a relatively high antibiotic resistance for Amoxicillin (100%), Erythromycin (98%), Cephradine (90%), Colistin (82%), Oxytetracyclin, (79%), Chloramphenicol (70%), Doxycycline (70%), and Kanamycin (62%) and high susceptibility for Imipenem (96%), Apramycin (94%), Amikacin (90%), Norfloxacin (78%), Sulphamethoxazol (86%), Enrofloxacin (64%), and Ofloxacin (60%). Furthermore, PCR was successfully amplified for the toxA, exoS, and oprL virulence genes at 396, 118, and 504 bp, respectively, as well as amplifying the ermB, pelA, blaTEM, and tetA resistance genes at 639, 786, 516, and 570 bp, respectively. The dendrogram investigation by ERIC-PCR of 10 clinical P. aeruginosa isolates revealed two main clusters and 10 different ERIC-PCR patterns. The presence of P. aeruginosa isolates in food may represent a potential public health concern, with the need for further epidemiological studies, as well as whole-genome sequencing and correlations of P. aeruginosa in water, food samples, and human infections.
Insights
This study identified Pseudomonas aeruginosa in human, water, and food samples in Egypt, revealing high antibiotic resistance and the presence of virulence genes. The findings highlight potential public health risks from contaminated food sources.
Area of Science:
- Microbiology
- Molecular Biology
- Public Health
Background:
- Pseudomonas aeruginosa is a significant cause of nosocomial and foodborne infections.
- Understanding the molecular epidemiology and antibiotic resistance of P. aeruginosa is crucial for public health.
- This study investigated P. aeruginosa in various environmental and food sources in Egypt.
Purpose of the Study:
- To perform molecular genotyping of P. aeruginosa isolates from human, water, and food specimens.
- To assess the antibiotic resistance patterns and identify virulence and resistance genes.
- To evaluate the genetic diversity of clinical P. aeruginosa isolates.
Main Methods:
- Sample collection from human, tap water, fish swamp, chicken meat, minced meat, raw milk, and hospital surfaces.
- Detection and isolation of P. aeruginosa, followed by antibiotic susceptibility testing.
- Polymerase Chain Reaction (PCR) for virulence (toxA, exoS, oprL) and resistance genes (ermB, pelA, blaTEM, tetA).
- Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR for genotyping clinical isolates.
Main Results:
- P. aeruginosa was detected in 14.28% of samples, with higher prevalence in human (26%) and water/food sources (10-18%).
- High resistance was observed for Amoxicillin (100%), Erythromycin (98%), and Cephradine (90%). High susceptibility was noted for Imipenem (96%) and Apramycin (94%).
- Virulence genes (toxA, exoS, oprL) and resistance genes (ermB, pelA, blaTEM, tetA) were successfully amplified.
- ERIC-PCR revealed 10 distinct patterns among 10 clinical isolates, indicating genetic diversity.
Conclusions:
- P. aeruginosa is prevalent in various sources in Egypt, posing a potential public health risk, especially through food contamination.
- The high rates of antibiotic resistance necessitate careful monitoring and infection control strategies.
- Further epidemiological studies and whole-genome sequencing are recommended to understand transmission dynamics.
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