Cheilanthenetetraol, a sesterterpenoid isolated from Aleuritopteris bicolor with anti-hepatocellular carcinoma and...

The ¹H-NMR, LC-MS, and HPLC guided isolation of n-hexane and ethyl acetate fractions led to the purification of one new sesterterpene cheilanthenetetraol (1) together with thirteen known compounds (2-14) for the first time from Aleuritopteris bicolor Roxb. The structure of the isolated compounds were established by extensive analysis of spectroscopic data, 1D and 2D NMR, HR-ESI-MS, and IR. The isolated compounds (2, 3, 6, and 9-14), as well as the n-hexane, ethyl acetate fractions, and aqueous methanol extract, were evaluated for cytotoxic activity against the HepG2 cancer cell line. All the tested compounds exhibited significant cytotoxic activity with an IC₅₀ ranging from 10.08 µM to 14.04 µM. The extracts exhibited moderate activity with an IC₅₀ value of 315.68, 391.55, and 274.89 µg/mL, respectively. The anti-inflammatory activity of compounds 6, 9, and 10 were assessed, and the results demonstrated that these compounds exhibited significant activity at 10 and 30 µM concentrations in LPS-stimulated macrophages.