Physiological adaptations to 6 weeks of high-intensity interval and moderate-intensity continuous training in horses:...

This study tested the hypothesis that 6 weeks of high-intensity interval training (HIIT) would induce greater physiological adaptations than moderate-intensity continuous training (MICT) in Thoroughbred horses. Seven untrained horses completed two distance-matched treadmill training protocols (three sessions per week) in a randomized crossover design, separated by a three-month washout: MICT (6 min at 70% $\dot{V}{O}_{2\max }$ ) and HIIT (6 × [30 s at 100% $\dot{V}{O}_{2\max }$ with 30 s at 30% $\dot{V}{O}_{2\max }$ ]). Incremental exercise tests were conducted at weeks 0, 3, and 6 to assess exercise performance and physiological responses. Mixed-effects models were used to analyze the effects of time, protocol, and their interaction (p < 0.05). After 6 weeks, HIIT elicited greater improvements than MICT in run distance to exhaustion (+29% vs. +4.5%), speed at $\dot{V}{O}_{2\max }$ (+9.8% vs. +2.4%), and speed at maximal heart rate (+15% vs. +4.3%). Speed at 10 mmol/L of plasma lactate increased only after HIIT (+13% vs. +6.0%). Both protocols similarly improved $\dot{V}{O}_{2\max }$ (+13%) and maximal cardiac output (+7%-8%). In conclusion, despite being matched for total running distance, HIIT induced superior improvements in performance, cardiovascular function, and lactate kinetics compared with MICT, highlighting training intensity as a key determinant of training adaptations in horses.