Related Experiment Video
Updated: Feb 19, 2026
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Published on: October 15, 2019
CleaveScope─An Easy Tool for Protease Specificity Analysis from LC-MS/MS Data
Mihail Konstantinov1, Ilya Toropygin1
1Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia.
Abstract:
Here we present CleaveScope, a lightweight standalone software tool for rapid and flexible analysis of protease specificity using LC-MS/MS data. The program accepts XML output files from the Mascot search engine, performs automated extraction and alignment of cleavage sites, and builds frequency matrices of amino acid residues surrounding scissile bonds. CleaveScope supports various normalization strategies, including direct normalization, percentage difference, and Z-score, enabling robust detection of amino acid preferences. The results are visualized as customizable heatmaps and sequence logos, which reflect statistically significant residue patterns around cleavage sites. The software is freely available at https://github.com/MiKonstantinov/CleaveScope.
More Related Videos
09:47The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
Published on: May 25, 2018
17:12Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
Published on: December 20, 2010
Related Concept Videos
01:33Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
01:20Mass Spectrometry: Molecular Fragmentation Overview
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...