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Sensitive Detection of Salmonella Susceptibility to Ceftriaxone and Azithromycin Using Matrix-Assisted Laser
Guohao Zhan1,2, Shujin Xie2,3, Jinyu Zhang4
1Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, Guangdong, China.
Background:
The rising incidence of Salmonella-induced diarrhea highlights the critical need for judicious antibiotic use to ensure effective treatment outcomes.
Methods:
This study presents an innovative methodology for evaluating the susceptibility of Salmonella to ceftriaxone and azithromycin through the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Results:
The proposed method requires only 2-3 individual Salmonella colonies and yields results within approximately 1 hour, assessing susceptibility to ceftriaxone (70 mg/L) and azithromycin (25 mg/L). In comparison to the Kirby-Bauer method, this technique exhibits a sensitivity of 98.64% and a specificity of 84.38%.
Conclusions:
The method developed in this study is both straightforward and user-friendly, making it suitable for deployment in major laboratories equipped with time-of-flight mass spectrometry. It offers a preliminary assessment of Salmonella susceptibility to ceftriaxone and azithromycin, thereby enhancing the accuracy of clinical interventions for Salmonella infections.
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