Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

4.3K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
4.3K
Structure-Activity Relationships and Drug Design01:28

Structure-Activity Relationships and Drug Design

1.9K
Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
1.9K
Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

704
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
704
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.8K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.8K
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

1.1K
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
1.1K
Adrenergic Agonists: Chemistry and Structure-Activity Relationship01:16

Adrenergic Agonists: Chemistry and Structure-Activity Relationship

3.9K
Adrenergic agonists' structure-activity relationship (SAR) determines their selectivity and efficacy. These agonists comprise a phenylethylamine moiety with an aromatic ring and an ethylamine side chain.
Aromatic ring substitutions: Substituting the aromatic ring with –OH groups at positions 3 and 4 yields catecholamines (e.g., epinephrine), which have a high affinity for adrenoceptors. Hydrogen bonding between –OH groups and receptors enhances adrenergic activity.
Separation of...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum for Theriault et al., "Utilization of a CRISPRi-based <i>ex vivo</i> challenge model to reveal temporally dependent gene essentiality in intracellular <i>Mycobacterium tuberculosis</i>".

mBio·2026
Same author

A deformylase inhibitor expands therapeutic options for Lyme disease.

Research square·2026
Same author

Utilization of a CRISPRi-based <i>ex vivo</i> challenge model to reveal temporally dependent gene essentiality in intracellular <i>Mycobacterium tuberculosis</i>.

mBio·2026
Same author

Cyclooctadiene-derived cage-divergent synthesis of heteroadamantanes and alternative polycyclic systems.

Organic & biomolecular chemistry·2026
Same author

A suite of macrocyclic peptide inhibitors and substrate probes for arginine methyltransferases.

Chemical science·2026
Same author

Energy expenditure and cellular activity underlie antibiotic tolerance of <i>Pseudomonas aeruginosa</i>.

mBio·2026

Related Experiment Video

Updated: Feb 19, 2026

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

9.1K

Structure-Activity Studies on the Antitubercular Natural Product Evybactin.

Vladyslav Lysenko1, Monique E Theriault2, Fabienne A C Sterk1

  • 1Biological Chemistry Group, Institute of Biology, Leiden University, 2333BE Leiden, The Netherlands.

ACS Medicinal Chemistry Letters
|February 18, 2026
PubMed
Summary

Researchers explored evybactin, a novel antibiotic targeting tuberculosis. Structure-activity relationship studies revealed key features essential for its potent antibacterial activity against Mycobacterium tuberculosis.

Keywords:
M. tuberculosisantibacterial resistanceevybactinstructure−activity studies

More Related Videos

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
10:41

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Published on: January 13, 2013

19.1K
Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments
07:50

Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments

Published on: October 25, 2024

2.4K

Related Experiment Videos

Last Updated: Feb 19, 2026

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

9.1K
The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
10:41

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Published on: January 13, 2013

19.1K
Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments
07:50

Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments

Published on: October 25, 2024

2.4K

Area of Science:

  • Medicinal Chemistry
  • Organic Synthesis
  • Microbiology

Background:

  • Antibiotic resistance is a major global health threat, necessitating the development of new drugs.
  • Evybactin, a depsipeptide antibiotic, shows selective activity against Mycobacterium tuberculosis.
  • Previous work established the total synthesis and revised structure of evybactin.

Purpose of the Study:

  • To investigate the structure-activity relationships (SAR) of evybactin.
  • To identify key structural components responsible for evybactin's antitubercular activity.
  • To guide the design of novel, more potent tuberculosis therapeutics.

Main Methods:

  • Synthesis of 21 novel evybactin analogues.
  • Systematic modifications including alanine scanning, macrocycle variations, N-terminus substitution, and side chain alterations.
  • Evaluation of the impact of structural changes on antitubercular activity.

Main Results:

  • Identified specific amino acids and structural features crucial for evybactin's potent activity.
  • Demonstrated that modifications to the ester-linked macrocycle and N-terminus significantly affect efficacy.
  • Highlighted the importance of positively charged side chains for optimal antibacterial function.

Conclusions:

  • Evybactin's unique structure is critical for its potent activity against Mycobacterium tuberculosis.
  • SAR studies provide a foundation for optimizing evybactin or designing new antitubercular agents.
  • This research contributes to the ongoing effort to combat drug-resistant tuberculosis.