High-throughput editing boosts the construction of next-generation microbial cell factories

Area of Science:

• Synthetic Biology
• Metabolic Engineering

Background:

• Traditional metabolic engineering struggles with inefficient gene editing, lengthy screening, and complex multi-gene optimization for microbial cell factories.
• Developing efficient microbial cell factories is crucial for advancing sustainable, bio-based production.

Purpose of the Study:

• To review high-throughput (HTP) genome editing mechanisms and applications in microbial metabolic engineering.
• To discuss HTP strategies that accelerate the design-build phase for microbial cell factories (MCFs).

Main Methods:

• Review of current high-throughput genome editing technologies.
• Analysis of HTP applications in metabolic pathway optimization and regulatory mechanism studies.

Main Results:

• HTP genome editing precisely and efficiently modifies multiple genes, overcoming traditional limitations.
• HTP enables rapid screening and modification of enzymes and regulatory factors across metabolic pathways.
• HTP facilitates both top-down analysis and bottom-up assembly of metabolic pathways.

Conclusions:

• HTP genome editing is a key enabling tool for constructing advanced microbial cell factories.
• These advancements promise to significantly enhance MCF performance for next-generation bio-production.
• HTP strategies are vital for accelerating the design-build cycle in metabolic engineering.