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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
Published on: March 28, 2015
Pathogen nucleic acids data in wastewater solids from 147 treatment plants in the United States: 2024-2025
Alexandria B Boehm1, Marlene K Wolfe2, Amanda L Bidwell1
1Department of Civil & Environmental Engineering, School of Engineering and Doerr School of Sustainability, 473 Via Ortega, Room 189, Stanford University, Stanford, CA, 94305, USA.
Abstract:
This data article provides human pathogen nucleic-acid concentrations in wastewater solids from 147 treatment plants across 40 states in the United States. Concentrations were measured up to 7 times a week at the plants. The data run from 1 July 2024 through 15 September 2025, and represents an extension and expansion of the measurements provided in a previous data article. Nucleic-acid concentrations were measured using droplet digital (reverse-transcription-) polymerase chain reaction (ddRT-PCR). This article provides concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A and B viruses, respiratory syncytial virus, human metapneumovirus, enterovirus D68, parvovirus B19, norovirus genotype II, rotavirus, Candida auris, hepatitis A virus, human adenovirus group F, mpox virus clade Ib, mpox virus clade II, H1, H3, and H5 influenza A virus, measles, and pepper mild mottle virus nucleic acids in wastewater solids. These data can be used to study infectious disease epidemiology.
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