Main characteristic metabolites with xanthine oxidase inhibitory activities from Yunnan Coffea arabica husks
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Updated: Feb 19, 2026
Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
Published on: October 2, 2018
1College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Abstract:
Coffee "husks", as primary by-products whose inadequate disposal causes environmental pollution, are rich in bioactive ingredients. The in-depth exploration and practical application of these by-products remain insufficient. A comprehensive investigation was performed with the combination of LC-MSn-IT-TOF analysis, antioxidant and xanthine oxidase (XO) inhibitory assays. A total of 55 main characteristic compounds were identified and their detailed mass spectrometry cleavage patterns were elucidated. Chlorogenic acid analogs as key bioactive constituents comprised over 66% of the identified phenols, which spontaneously bind to XO, with binding energy ranging from -4.46 to -3.32 kcal/mol. The antioxidant activities of di-substituted chlorogenic acids were significantly higher than those of mono-substituted molecules. Flavonoids from the "husks" with XO inhibitory activities were attributed to the flavone skeleton, in which the presence of a stable chromone group was vital. These results provided novel perspectives for discovery of XO inhibitors from widely discarded natural by-products like coffee "husks".
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