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Updated: Jul 17, 2026

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP
06:36

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

Published on: April 9, 2013

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In vitro antibacterial activity of platelet-rich plasma against Gram-positive and Gram-negative pathogens

Yan Liu1, Lei Liu2,3, Jinbing Du2

  • 1Department of Clinical Laboratory, Maternal and Child Health Hospital of Hubei Province, Wuhan, Hubei, China.

Frontiers in Cellular and Infection Microbiology
|February 18, 2026
PubMed

Insights

Platelet-rich plasma (PRP) exhibits antibacterial properties against common wound pathogens. While PRP shows stronger antimicrobial activity than frozen PRP (Fro-PRP) and platelet-poor plasma (PPP), all demonstrated effectiveness, suggesting potential for infected wound treatment.

Area of Science:

  • Biomedical Science
  • Microbiology
  • Wound Healing Research

Background:

  • Platelet-rich plasma (PRP) is an autologous biological agent known to promote wound healing.
  • Bacterial infections frequently complicate wounds, hindering the healing process.
  • The precise effect of PRP on bacteria remains inconclusive, necessitating further investigation.

Purpose of the Study:

  • To evaluate the in vitro antibacterial activity of PRP, platelet-poor plasma (PPP), and frozen PRP (Fro-PRP).
  • To assess the antimicrobial effects against common wound pathogens: Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Staphylococcus aureus, and Enterococcus faecalis.

Main Methods:

  • Utilized a modified agar diffusion method to assess antimicrobial activity.
  • Employed colony-forming units counting test for quantitative bacterial analysis.
  • Conducted bacterial growth curve analysis to monitor microbial proliferation over time.

Main Results:

  • PRP, PPP, and Fro-PRP demonstrated antimicrobial effects against all five tested bacterial strains.
  • The antimicrobial activity of PRP was significantly more potent compared to Fro-PRP and PPP.
  • Frozen storage appeared to diminish the antibacterial capabilities of PRP to some extent.

Conclusions:

  • PRP, PPP, and Fro-PRP possess inherent antimicrobial properties against common wound bacteria.
  • PRP exhibits superior antibacterial efficacy compared to its frozen counterpart and PPP.
  • PRP and other platelet-derived products represent a promising therapeutic avenue for managing infected wounds.
Keywords:
antibacterial activityfrozen platelet-rich plasmainfected woundsplatelet-poor plasmaplatelet-rich plasma

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