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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Related Experiment Video

Updated: Feb 20, 2026

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
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Comparing Liquid Vortex Capture & the Rapid Droplet Sampling Interface for Single Cell Mass Spectrometry.

Stephen C Zambrzycki1, Vilmos Kertesz1, John F Cahill1

  • 1Bioanalytical Mass Spectrometry Group, Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, Tennessee 37831-6131, United States.

Journal of the American Society for Mass Spectrometry
|February 18, 2026
PubMed
Summary
This summary is machine-generated.

Rapid Droplet Sampling Interface (RDSI) significantly enhances single-cell mass spectrometry sensitivity for drugs and lipids. Liquid Vortex Capture (LVC) offers better reproducibility with higher sample volumes, balancing speed and reliability in metabolomics.

Keywords:
inkjet dispensinglipidomicsliquid vortex captureopen port sampling interfacepharmaceuticalsrapid droplet sampling interfacesingle-cell mass spectrometry

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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • High-throughput single-cell mass spectrometry demands advanced sampling and ionization methods.
  • Balancing speed, sensitivity, and reliability is crucial for metabolomic and lipidomic analyses.

Purpose of the Study:

  • To comparatively analyze the performance of Liquid Vortex Capture (LVC) and Rapid Droplet Sampling Interface (RDSI) for single-cell mass spectrometry.
  • To benchmark these platforms using pharmaceuticals, EquiSPLASH, and single-cell experiments.

Main Methods:

  • Performance evaluation of LVC and RDSI ionization platforms.
  • Benchmarking using pharmaceutical compounds (propranolol, amiodarone, atorvastatin), lipids (phosphocholines), and HepG2 cell experiments.
  • Analysis of sensitivity, reproducibility, and flow rate effects.

Main Results:

  • RDSI achieved up to 100-fold sensitivity improvement for drugs and lipids in solution due to low-flow rates (3 μL/min) and reduced dilution.
  • LVC demonstrated superior reproducibility with higher flow rates (200 μL/min), accommodating larger volumes and improving solubility/cleaning.
  • RDSI showed up to 85-fold sensitivity enhancement for intracellular drugs and lipids in HepG2 cells.

Conclusions:

  • RDSI offers significant sensitivity gains for single-cell drug monitoring and lipidomics.
  • LVC provides enhanced reproducibility for higher throughput applications.
  • The choice between RDSI and LVC depends on the specific requirements for sensitivity, sample volume, and reproducibility in single-cell mass spectrometry.