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SlNAC63-SlbHLH71 module enhances tomato saline-alkali tolerance via regulating JA biosynthesis and ROS scavenging.

Xiangguang Meng1,2,3, Zhen Kang1,2,3, Xiaoyan Liu1,2,3

  • 1College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
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PubMed
Summary

Soil salinization harms crops. This study reveals how the SlNAC63-SlbHLH71 module enhances tomato tolerance to saline-alkali stress by boosting jasmonic acid (JA) and reactive oxygen species (ROS) scavenging.

Keywords:
SlNAC63Jasmonic acid synthesisROS scavengingSaline-alkaliTomato

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Agricultural Science

Background:

  • Soil salinization is a major agricultural constraint, limiting plant growth and crop yield worldwide.
  • NAC transcription factors are known regulators of plant responses to abiotic stresses.
  • Understanding the molecular mechanisms of plant stress tolerance is crucial for developing resilient crops.

Purpose of the Study:

  • To investigate the role of SlNAC63 in tomato (Solanum lycopersicum L.) response to saline-alkali stress.
  • To elucidate the molecular mechanism by which SlNAC63 regulates saline-alkali tolerance, focusing on jasmonic acid (JA) signaling and reactive oxygen species (ROS) scavenging.
  • To identify and characterize interacting partners of SlNAC63 involved in stress response.

Main Methods:

  • Yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) to confirm direct gene targets.
  • Overexpression of SlAOS1 and SlSOD4 in tomato to assess their roles in JA accumulation and ROS scavenging.
  • Yeast two-hybrid (Y2H), pull-down, and co-immunoprecipitation (Co-IP) assays to identify protein-protein interactions.
  • Phenotypic analysis of transgenic tomato plants under saline-alkali stress.

Main Results:

  • SlNAC63 expression is induced by saline-alkali stress and jasmonic acid (JA).
  • SlNAC63 directly targets and regulates the expression of SlAOS1 (involved in JA biosynthesis) and SlSOD4 (involved in ROS scavenging).
  • Overexpression of SlAOS1 increased JA accumulation, while SlSOD4 overexpression enhanced ROS scavenging under stress.
  • SlNAC63 interacts with SlbHLH71, and this interaction enhances SlNAC63's binding affinity to the promoters of SlAOS1 and SlSOD4, thereby strengthening saline-alkali tolerance.
  • The SlNAC63-SlbHLH71 module positively regulates saline-alkali tolerance by modulating JA biosynthesis and ROS detoxification.

Conclusions:

  • The SlNAC63-SlbHLH71 module plays a central role in tomato's response to saline-alkali stress.
  • This module enhances plant tolerance by coordinating JA accumulation and ROS scavenging pathways.
  • The findings provide a molecular basis for improving crop resilience to soil salinization through genetic engineering.