Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Impact of OnabotulinumtoxinA on Oral Pain Medication Prescription Fills and Low-Value Care in Patients with Cervical Dystonia in the United States: A Retrospective Claims Analysis.

Toxins·2026
Same author

Real-World Treatment Switch Rates, and Healthcare Costs of Botulinum Toxin Type A Among Patients with Cervical Dystonia in the USA: A Retrospective Claims Analysis.

Neurology and therapy·2026
Same author

Real-World Switch Rates, Treatment Patterns, and Healthcare Costs Among Patients With Spasticity Treated With Botulinum Toxins.

Advances in therapy·2026
Same author

Electroencephalography signals in a female Fragile X Syndrome mouse model.

NeuroImage·2026
Same author

A study to evaluate the impact of PB-119 injection (a pegylated exenatide formulation) on the pharmacokinetic profiles of Digoxin and Warfarin sodium in healthy subjects.

SAGE open medicine·2026
Same author

Alterations in electroencephalography signals in female fragile X syndrome mouse model on a C57BL/6J background.

Physiology & behavior·2026

Related Experiment Video

Updated: Jun 28, 2026

A Mouse Model of in Utero Transplantation
09:11

A Mouse Model of in Utero Transplantation

Published on: January 27, 2011

20.1K

Single Intraperitoneal Busulfan Injection Induces Long-Term Reproductive Dysfunction and Reduces Male Offspring Ratio

Ruiyang Pu1, Xiaotong Sun1, Wei Zhang2

  • 1Department of Obstetrics, Gansu Provincial Hospital, The Third Hospital of Lanzhou University (The Third Clinical College), Lanzhou, Gansu, China.

Journal of Applied Toxicology : JAT
|February 18, 2026
PubMed
Summary
This summary is machine-generated.

Busulfan exposure significantly impairs male mouse fertility, reducing sperm quality and offspring sex ratio for at least 13 weeks. This study highlights potential transgenerational reproductive toxicity from busulfan treatment.

Keywords:
busulfanfertility impairmentfertility trialmouse modelsemen analysis

More Related Videos

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
11:25

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Published on: April 1, 2015

14.1K
A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
07:48

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice

Published on: October 13, 2018

8.1K

Related Experiment Videos

Last Updated: Jun 28, 2026

A Mouse Model of in Utero Transplantation
09:11

A Mouse Model of in Utero Transplantation

Published on: January 27, 2011

20.1K
Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
11:25

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Published on: April 1, 2015

14.1K
A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
07:48

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice

Published on: October 13, 2018

8.1K

Area of Science:

  • Reproductive Toxicology
  • Toxicology
  • Genetics

Background:

  • Busulfan is a chemotherapeutic agent with known reproductive side effects.
  • Understanding its long-term toxicological impact on male fertility is crucial for risk assessment.

Purpose of the Study:

  • To evaluate the sustained effects of busulfan on male reproductive function and fertility in BALB/c mice.
  • To investigate potential transgenerational impacts on offspring, including sex ratio alterations.

Main Methods:

  • BALB/c mice received single intraperitoneal injections of busulfan (15, 20, or 25 mg/kg).
  • Spermatogenesis, semen quality, and fertility were assessed at multiple time points.
  • Fertility trials and offspring sex ratio analysis were conducted.

Main Results:

  • Busulfan significantly decreased relative testicular weight and semen quality (p < 0.05).
  • Abnormal sperm morphology increased, correlating with reduced motility (p < 0.05).
  • Busulfan-treated males showed reduced fertility, lower pregnancy rates, and smaller litter sizes, with a skewed male offspring ratio.

Conclusions:

  • A single busulfan dose causes prolonged male reproductive impairment (≥13 weeks) in mice.
  • Busulfan exposure may induce transgenerational effects, altering the sex ratio of male descendants.