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Bioparticle concentration in operating room air: a comparison between two clothing systems of different source
L Larsson1, J Nordenadler2, L Felländer-Tsai3
1Division of Orthopaedics and Biotechnology, CLINTEC, Karolinska Institutet, Stockholm, Sweden; Department of Perioperative Medicine and Intensive Care, Karolinska University Hospital, Stockholm, Sweden.
Background:
The permeability of surgical scrubs affects the bacterial load in the operating room (OR) air. Bacterial load can be measured by counting fluorescent bioparticles per unit of air. This study aimed to compare fluorescent bioparticle sized ≥3 μm per 50 dm3 of OR air (FBP) in air during primary arthroplasty surgeries using two different staff clothing systems.
Methods:
The average FBP for 37 consecutive arthroplasty surgeries using reusable scrubs was compared with 37 surgery type-matched arthroplasties using single-use scrubs. An analysis of covariance (ANCOVA) was conducted to analyse the effects of surgery duration on log10-transformed FBP while controlling for the number of staff members present and surgery duration.
Findings:
Mean FBP levels were 101 (95% confidence interval [CI]: 96-107) for the reusable scrubs and 18 (95% CI:16-20) for the single-use scrubs. In the ANCOVA, clothing type was strongly associated with log10-transformed FBP, accounting for 86% of the partial variance (η2 = 0.86). In contrast, neither the number of staff nor surgery duration had a significant effect on FBP.
Conclusion:
Clothing system permeability to bioparticles is one key factor in achieving air with low levels of bioparticles in OR air. Measuring FBP in real time during arthroplasty surgery is a new possibility for studying the effect of distinct OR-related factors on air bacterial load.
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