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Substrate secretion by different EHEC secretion systems during their interaction with epithelial cells.

Landy Zambrano-Arguello1, Jaime Vazquez-Lopez1, Fernando Navarro-Garcia1

  • 1Department of Cell Biology, Centro de Investigación y de Estudios Avanzados (Cinvestav), Mexico City, Mexico.

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|February 19, 2026
PubMed
Summary
This summary is machine-generated.

Enterohemorrhagic Escherichia coli (EHEC) uses multiple secretion systems (SSs) that interact during infection. Epithelial cells influence EHEC

Keywords:
EHEC-epithelial cells interactionEscherichia colibacterial effectorsprotein secretionproteomicssecretion systemsvirulence factors

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Area of Science:

  • Microbiology
  • Pathogenesis
  • Bacterial Secretion Systems

Background:

  • Enterohemorrhagic Escherichia coli (EHEC) is a significant cause of human foodborne illness.
  • EHEC possesses five types of secretion systems (SSs), but their collective role in host-pathogen interaction is poorly understood.
  • While some substrates for these SSs are known, their coordinated function during infection remains largely unexplored.

Purpose of the Study:

  • To investigate the collective function of EHEC secretion systems during interaction with epithelial cells.
  • To elucidate the hierarchy and interdependence of protein secretion via different SSs in EHEC.
  • To identify novel substrates secreted by EHEC SSs and understand their role in pathogenesis.

Main Methods:

  • Optimized protein secretion assays for four EHEC SSs in vitro and in the presence of epithelial cells.
  • Utilized Western blot and proteomics to analyze EHEC secretome changes.
  • Performed bioinformatics analyses to predict new SS substrates.
  • Generated and analyzed mutants deficient in specific SSs.

Main Results:

  • Epithelial cells significantly increased protein secretion by EHEC, influencing the abundance of specific substrates.
  • A hierarchical secretion pattern was observed when EHEC encountered epithelial cells.
  • Mutations in one SS impacted the protein secretion of other SSs, indicating interdependence.
  • Epithelial cells modulated the effects of SS mutations, and new potential substrates were identified.

Conclusions:

  • A complex interplay exists between EHEC secretion systems during epithelial cell infection.
  • This SS interaction influences host-pathogen interactions and EHEC pathogenesis.
  • Understanding these SSs is crucial for developing strategies against EHEC infections.