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Related Experiment Video

Updated: Jun 16, 2026

Identification and Dissection of Diverse Mouse Adipose Depots

Identification and Dissection of Diverse Mouse Adipose Depots

Published on: July 12, 2019

A Sensitive Reporter Mouse Model to Study Adipocyte-Derived Extracellular Vesicles In Vivo.

Didde Riisager Hansen^1,2, Rugivan Sabaratnam^1,2,3, Lasse Bach Steffensen¹

¹Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark.

Journal of Extracellular Vesicles

|February 19, 2026

Summary

Researchers developed a sensitive in vivo reporter system using adeno-associated virus (AAV) vectors to track adipocyte-derived extracellular vesicles (EVs). This system revealed early high-fat diet effects on EV distribution in mice.

Keywords:

CD63 CD9 Cre recombinase EV reporter adipose tissue nanoluciferase obesity

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Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro

Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro

Published on: December 6, 2025

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Extracellular vesicles (EVs) play crucial roles in intercellular communication in vivo.
Studying cell type-specific EV abundance and distribution in biological fluids presents significant technical challenges.

Purpose of the Study:

To develop a sensitive in vivo reporter system for monitoring cell type-specific EVs, focusing on adipocyte-derived EVs.
To investigate the impact of a high-fat diet (HFD) on adipocyte-derived EVs using the developed reporter system.

Main Methods:

Generated adeno-associated virus (AAV) vectors encoding Cre-activated CD63-NanoLuc (CD63-NanoLuc) and constitutive sec-NanoLuc reporters.
Administered AAVs to mice to achieve adipocyte-specific reporter expression.
Measured NanoLuc activity in plasma, urine, and tissues of mice under normal and HFD conditions.

Main Results:

Adipocyte-specific expression of both reporters was successfully induced by AAV administration.
CD63-NanoLuc activity was primarily detected in adipose tissues, while sec-NanoLuc was found in plasma and urine.
HFD significantly increased CD63-NanoLuc activity in adipose tissues, lungs, kidneys, and urine, indicating altered adipocyte-derived EV release and distribution.

Conclusions:

The CD63-NanoLuc and sec-NanoLuc reporter systems provide a sensitive method for monitoring cell type-specific EVs in vivo.
The study demonstrates an early effect of HFD on adipocyte-derived EV abundance and distribution, detectable by the reporter system.
This system offers a valuable tool for studying EVs in various physiological and pathological conditions.