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Updated: Feb 21, 2026

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Published on: November 12, 2015
RACK1 inhibits fowl adenovirus serotype 4 replication by targeting the viral protein Hexon for ubiquitin-proteasome
Wei Li1, Hongnuan Wang1, Zhen Yan1
1Shanxi Key Laboratory of Animal Disease Research, Prevention and Control, College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China.
Abstract:
Fowl adenovirus serotype 4 (FAdV-4), the most pathogenic member of the genus Aviadenovirus, within the family Adenoviridae, primarily induces hepatitis-hydropericardium syndrome (HHS), leading to high morbidity and mortality in chickens. The capsid protein Hexon has been established as a core virulence factor of FAdV-4, but the underlying interaction mechanisms between Hexon and the host remain to be fully elucidated. In this study, we identified that the host protein RACK1 endogenously interacts with the Hexon, and that overexpression of RACK1 significantly inhibited FAdV-4 replication, whereas knockdown of RACK1 markedly promoted this process. Mechanistically, the interaction between RACK1 and Hexon promotes the degradation of Hexon via the ubiquitin-proteasome pathway, thus suppressing FAdV-4 replication. Our results further enrich the understanding of the interaction between Hexon and the host and identifies a novel host factor interaction that may inform future strategies for controlling HHS.
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