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Recombinant live biotherapeutics: A new frontier in peptide drug biosynthesis and precision delivery
Meenal Chawla1, Omkar Bhange2, Bhabatosh Das1
1Functional Genomics Laboratory, BRIC-Translational Health Science and Technology Institute, Microbial Research Centre, NCR Biotech Science Cluster, Faridabad, Haryana, India.
Abstract:
Peptide-based therapeutics are widely employed in the treatment and management of various medical conditions, including diabetes, obesity, cancer, rare diseases, and microbial infections. This chapter reviews the evolution of technologies used in peptide production including chemical synthesis, recombinant DNA technology, and other advanced methods that have driven significant progress in pharmaceutical development. However, the development of peptide therapeutics is challenged by issues related to high production costs, limited stability, delivery barriers, and potential toxicity. To overcome these issues, recombinant live biotherapeutics (rLBPs), can be utilized as live microbioreactor, where genetically engineered microorganisms are employed to produce and deliver therapeutic peptides directly within the host. Recombinant LBPs enable accurate, sustained, and targeted delivery of therapeutic peptides with enhanced safety, efficacy and cost-effective manufacturing. Furthermore, this chapter highlights the advancements of genetic engineering tools that have enabled the modification and development of rLBPs as microbioreactor, highlighting their emerging role and therapeutic potential in curing disorders.
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