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Updated: Jul 8, 2026

Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes
Published on: February 13, 2019
Rational design and multidimensional profiling of polyfluorinated pyrethroids: a resistance-breaking strategy for
Xiaofang Li1, Liwen Lei1, Xu Wang1
1School of Pharmacy and Food Engineering, Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Wuyi University, Jiangmen, People Republic of China.
Background:
Pyrethroids based on chrysanthemic acid are widely used for mosquito control due to their high potency and low mammalian toxicity. However, growing resistance and environmental concerns necessitate new analogues. This study designed novel polyfluorinated pyrethroids derived from chrysanthemic acid scaffolds to overcome resistance, reduce ecotoxicity, and improve photostability.
Results:
Fourteen of 54 synthesized chrysanthemate-based compounds exceeded bifenthrin (LC50 = 1.96 mg/L) against sensitive mosquitoes. Lead compounds BFA-3-1, BFA-3-2, and BFA-3-4 showed LC50 values of 0.55, 0.45, and 0.53 mg/L, respectively, and were 5-7 times more potent against resistant strains than deltamethrin. They also exhibited 10-fold lower acute toxicity to zebrafish (96 h-LC₅₀ = 0.47-0.67 mg/L) and longer photodegradation half-lives (108-133 min under simulated sunlight).
Conclusion:
Chrysanthemic acid-based polyfluorinated pyrethroids effectively break resistance, reduce non-target toxicity, and maintain photostability, offering a sustainable chemical template for next-generation vector control. © 2026 Society of Chemical Industry.
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