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Related Experiment Video

Updated: Feb 21, 2026

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels
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3D coaxial bioprinting of RADA16-I self-assembling peptide hydrogel.

M Jergitsch1,2, S Perez-Amodio1,2, L M Delgado1,2

  • 1Bioengineering Institute of Technology, Universitat Internacional de Catalunya, St/ Josep Trueta s/n, Sant Cugat del Vallès, Barcelona, 08195, Spain.

Materials Today. Bio
|February 20, 2026
PubMed
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This study introduces coaxial extrusion 3D bioprinting of RADA16-I peptide hydrogels, overcoming mechanical limitations for biofabrication. The method successfully printed stable, cell-laden scaffolds with high viability, paving the way for advanced tissue engineering applications.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Biofabrication

Background:

  • Self-assembling peptide hydrogels offer dynamic cell microenvironments but have limited mechanical properties for 3D bioprinting.
  • Extrusion 3D bioprinting requires materials with sufficient mechanical stability, which challenges the use of soft peptide hydrogels.

Purpose of the Study:

  • To develop a coaxial extrusion 3D bioprinting strategy for RADA16-I peptide hydrogels.
  • To enhance the printability and structural integrity of soft peptide hydrogels using a composite shell.
  • To evaluate the viability and behavior of encapsulated mesenchymal stem cells (MSCs) within the bioprinted scaffolds.

Main Methods:

  • Utilized a coaxial 3D bioprinter to co-extrude a RADA16-I peptide core (with methylcellulose and sucrose) and an MC-alginate hydrogel shell.
Keywords:
Coaxial 3D extrusion bioprintingSelf-assembling peptide hydrogelTissue engineering

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  • Characterized the rheological properties of the core solution to ensure printability without compromising self-assembly.
  • Assessed scaffold shape fidelity, structural integrity over 21 days, and cell viability and matrix deposition of encapsulated MSCs.

    • Main Results:

    • The coaxial extrusion method successfully printed RADA16-I peptide hydrogels with enhanced mechanical stability provided by the MC-alginate shell.
    • Printed scaffolds exhibited excellent shape fidelity and structural integrity throughout the 21-day culture period.
    • Encapsulated MSCs maintained high viability (>90%) and showed signs of aggregation, collagen network formation, and calcium phosphate deposition.

    Conclusions:

    • Coaxial extrusion 3D bioprinting is a viable strategy for fabricating constructs using mechanically soft, self-assembling peptide hydrogels.
    • This approach overcomes the limitations of peptide hydrogel mechanical properties for extrusion-based biofabrication.
    • The developed method holds promise for creating advanced, cell-laden scaffolds for tissue engineering and regenerative medicine applications.