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RUNX1 promotes pathological retinal angiogenesis through von Willebrand factor.

Ye Liu1,2, Chenfeng He3, Xiaogang Luo1,2

  • 1Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang Provincial Key Laboratory of Ophthalmology, Hangzhou, China.

Advances in Ophthalmology Practice and Research
|February 20, 2026
PubMed
Summary
This summary is machine-generated.

Runt-related transcription factor 1 (RUNX1) inhibition targets pathological retinal angiogenesis by modulating protein expression, particularly von Willebrand factor (vWF). This study reveals RUNX1’s mechanism in angiogenesis via the vWF axis.

Keywords:
ECM-receptor interactionRUNX1 inhibitionRetinal neovascularizationVon Willebrand factor

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Proteomics

Background:

  • Runt-related transcription factor 1 (RUNX1) is crucial for angiogenesis.
  • Pharmacological inhibition of RUNX1 can reduce pathological retinal angiogenesis.
  • The precise protein-level mechanisms of RUNX1 in promoting angiogenesis are not fully understood.

Purpose of the Study:

  • To investigate the functional mechanisms of RUNX1 in promoting angiogenesis at the protein level.
  • To identify downstream protein targets of RUNX1 involved in retinal pathological angiogenesis.
  • To evaluate RUNX1 inhibition as a therapeutic strategy for retinal angiogenesis.

Main Methods:

  • Utilized oxygen-induced retinopathy (OIR) and RUNX1 inhibition models in mice.
  • Analyzed retinal proteomes using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in Data-Independent Acquisition (DIA) mode.
  • Conducted in vitro experiments to assess the impact of von Willebrand factor (vWF) on endothelial cell migration and sprouting.

Main Results:

  • Identified 465 differentially expressed proteins (DEPs), with 295 upregulated and 170 downregulated.
  • Bioinformatic analysis highlighted enrichment in pathways like ECM-receptor interaction and focal adhesion.
  • RUNX1 inhibition reversed the dysregulation of 57 proteins, strongly associated with ECM-receptor interaction signaling. Von Willebrand factor (vWF) was identified as a downstream target of RUNX1.

Conclusions:

  • RUNX1 plays a significant role in retinal pathological angiogenesis.
  • RUNX1's pro-angiogenic effects are mediated through the von Willebrand factor (vWF) axis.
  • RUNX1 represents a promising therapeutic target for treating retinal pathological angiogenesis.