Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of nitrate-dependent Fe(II)-oxidizing bacteria in coupling nitrogen and phosphorus cycling in nearshore sediments of shallow lakes.

Water research·2026

Same author

Multidimensional regulatory mechanisms of chicken immunity: focus on viral infection, intestinal microbiota and heat stress.

Frontiers in veterinary science·2026

Same author

Multimodal Information-Driven Heterogeneous Graph Neural Networks for Protein-Ligand Binding Affinity Prediction.

Journal of chemical information and modeling·2026

Same author

Biomechanical differences between occipital plate and modified C1 lateral mass screw in the treatment of complex craniocervical malformations: a finite element analysis.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026

Same author

Tafenoquine: A Breakthrough Option for Babesiosis Treatment.

Tropical medicine and infectious disease·2026

Same author

ROS-responsive hydrogel microspheres deliver shTMEM2-loaded liposomes to suppress pyroptosis and ameliorate intervertebral disc degeneration.

Journal of nanobiotechnology·2026

Same journal

Frontier Advances of Terpyridine-Zn(II) Complexes: From Molecular Design to Smart Functional Materials.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

CREAT: A CRISPR-Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Metal (n+1)p-nd Orbital Hybridization and Excited-State Metal-Ligand π-Interactions Enable d<sup>10</sup> Carbene-Metal-Amide TADF OLEDs with High Efficiency and Long Operational Lifetime.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Thermoelectric PANI/TeNWs Fiber Based Microsensor for Passive Temperature and Active Chemical Sensing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Expanded Hepatic Progenitor Cells Featured with Aggregation of α-Synuclein Contribute to Pathologic Bile Duct Regeneration in Biliary Atresia.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Sub-Terahertz Memristor Switches Using MoS<sub>2</sub> by Liquid-Liquid Interface Assembly.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 22, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

vEMRec: High-Resolution Volume Electron Microscopy Reconstruction Based on Structure-Preserving and High-Fidelity 3D

Zhenbang Zhang^1,2,3, Hongjia Li¹, Zhongjun Yang⁴

¹School of Medical Technology, Beijing Institute of Technology, Beijing, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|February 20, 2026

Summary

This summary is machine-generated.

vEMRec, a novel algorithm for volume electron microscopy (vEM), accurately aligns 3D data by separating alignment into high and low frequencies. This method corrects distortions while preserving biological variations, improving downstream analysis.

Keywords:

deep learning series section electron microscopy volume electron microscopy

More Related Videos

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

Related Experiment Videos

Last Updated: Feb 22, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

Area of Science:

Microscopy and Imaging Science
Computational Biology
Bioinformatics

Background:

Accurate three-dimensional (3D) alignment is crucial for volume electron microscopy (vEM) to reconstruct biological structures.
Automated 3D alignment faces challenges in balancing distortion correction with preserving natural biological variations.

Purpose of the Study:

To introduce vEMRec, a fully automated algorithm for vEM 3D alignment.
To address the limitations of existing methods in handling nonlinear distortions and morphological variations.

Main Methods:

vEMRec redefines 3D alignment by decoupling it into high-frequency and low-frequency subproblems.
Employs precision rigid alignment for rigid distortions and Gaussian filter-driven elastic registration for nonlinear distortions.
Preserves biologically plausible deformations during the alignment process.

Main Results:

vEMRec demonstrates a paradigm shift in 3D alignment accuracy and efficiency.
Significantly enhances downstream tasks like isotropic reconstruction and 3D segmentation by improving axial continuity.
Preserves the integrity of ultrastructural details in large-scale datasets (TB-scale).

Conclusions:

vEMRec offers an accurate, robust, and efficient solution for large-scale vEM data processing.
The algorithm successfully optimized six diverse, large-scale real-world datasets.
vEMRec enables biologically relevant throughput for TB-scale specimen analysis.