Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "Thiolate DNAzymes on Gold Nanoparticles for Isothermal Amplification and Detection of Mesothelioma-derived Exosomal PD-L1 mRNA".

Analytical chemistry·2026
Same author

Thigmotactic navigation and functional enrichment of boar sperm in microfluidic channels.

Reproduction (Cambridge, England)·2026
Same author

Sperm DNA fragmentation and its influence on mammalian reproduction.

Nature reviews. Urology·2026
Same author

CRISPR/Cas9-mediated gene editing in trophoblast cells via mechanoporation for preeclampsia insight.

Cell death & disease·2025
Same author

Role of exosomes in cancer: from diagnosis to therapy.

Discover oncology·2025
Same author

<i>Giardia</i> purification from fecal samples using rigid spiral inertial microfluidics.

Biomicrofluidics·2025
Same journal

Umbilical Cord Blood-Derived Extracellular Vesicles Enhance Neurovascular Regeneration and Improve Erectile Function in a Mouse Model of Bilateral Cavernous Nerve Injury.

Andrology·2026
Same journal

Proteomic Profiling of Cryopreservation-Induced Alterations in Rhesus Macaque Sperm.

Andrology·2026
Same journal

Sperm Head-Tail Coupling Apparatus Diversity and Common Themes Among Species.

Andrology·2026
Same journal

Intrauterine Growth and Male Reproductive Health: A Follow-up Study From the Danish National Birth Cohort.

Andrology·2026
Same journal

Antioxidants and Nutraceutical for Male Factor Infertility: Where Does the Evidence Stand?

Andrology·2026
Same journal

Can Hormonal Therapy Improve the Outcomes of mTESE in Patients With Non-Obstructive Azoospermia?

Andrology·2026
See all related articles

Related Experiment Video

Updated: Feb 22, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
09:16

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

697

Microfluidic Intracytoplasmic Sperm Injection (MICSI): A Novel Platform for Sperm Isolation, Selection, and Injection

Mehran Dabiri1, Dale Mark Goss1,2, Steven Alexander Vasilescu1

  • 1School of Biomedical Engineering, Faculty of Engineering and IT, University of Technology Sydney, Sydney, New South Wales, Australia.

Andrology
|February 20, 2026
PubMed
Summary
This summary is machine-generated.

A new microfluidic sperm selection device (MICSI) offers improved sperm quality for assisted reproduction. This technology enhances motility and reduces DNA damage compared to traditional methods, potentially improving IVF success rates.

Keywords:
ICSIdensity gradient centrifugationhyaluronic acidmicrofluidicssperm selection

More Related Videos

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

12.3K
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

23.6K

Related Experiment Videos

Last Updated: Feb 22, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
09:16

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

697
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

12.3K
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

23.6K

Area of Science:

  • Reproductive Medicine
  • Biotechnology
  • Microfluidics

Background:

  • Infertility affects 1 in 6 couples globally, with high assisted reproductive technologies (ART) failure rates.
  • Current sperm processing methods like density gradient centrifugation (DGC) have limitations, including potential DNA damage.
  • Technological innovation in sperm processing is crucial for improving ART outcomes.

Purpose of the Study:

  • To introduce and evaluate a novel microfluidic Intracytoplasmic Sperm Injection (MICSI) platform.
  • To integrate semen processing, sperm selection (motility and hyaluronic acid binding), and oocyte insemination into a single device.
  • To compare the performance of the MICSI device against conventional DGC methods.

Main Methods:

  • Developed a microfluidic ICSI (MICSI) platform integrated into standard ICSI dishes.
  • Evaluated sperm parameters including DNA fragmentation index (DFI), motility, and morphology.
  • Compared MICSI performance against density gradient centrifugation (DGC) and DGC plus hyaluronic acid (HA) binding.

Main Results:

  • The MICSI device successfully selected sperm with significantly higher progressive motility (p < 0.0001).
  • MICSI-selected sperm exhibited significantly better morphology (p < 0.0001) and lower DFI (p < 0.0001) compared to raw semen.
  • Compared to DGC, MICSI yielded sperm with superior motility and lower DFI (p < 0.0001).

Conclusions:

  • The MICSI device demonstrates superior performance in selecting high-quality spermatozoa.
  • This microfluidic platform offers a promising clinical alternative for sperm selection in ART.
  • MICSI may enhance ART success rates by providing a more effective sperm preparation method.