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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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Author Spotlight: Exploring the Impact of Trauma on Cellular Aging
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Improved Step-by-Step qPCR Method for Absolute Telomere Length Measurement.

Ekaterina Sergeevna Arshinova1, Nataliia Sergeevna Karpova1, Olga Leonidovna Terekhina1

  • 1Federal State Budgetary Institution "Research Institute of Pathology and Pathophysiology", 125315 Moscow, Russia.

Methods and Protocols
|February 20, 2026
PubMed
Summary

This study presents an improved quantitative PCR (qPCR) method for precise absolute telomere length measurement. The validated technique offers a rapid, cost-effective solution for cellular aging and disease research.

Keywords:
biological agingmethodprotocolquantitative PCRtelomeretelomere length

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Area of Science:

  • Genetics
  • Molecular Biology
  • Biochemistry

Background:

  • Telomere length is a critical biomarker for cellular aging and genomic stability.
  • Accurate and high-throughput telomere length measurement is essential for research and clinical diagnostics.
  • Existing methods may lack precision or scalability for large-scale studies.

Purpose of the Study:

  • To introduce an improved quantitative PCR (qPCR) method for measuring absolute telomere length.
  • To establish a reliable and efficient assay for telomere length quantification.
  • To facilitate advancements in the study of age-related diseases and cancers.

Main Methods:

  • Developed novel primers for the single-copy gene interferon beta (IFNB1) as an internal control.
  • Utilized single-stranded oligonucleotide standards to create a calibration curve for precise quantification.
  • Validated the method using DNA from peripheral blood, buccal swabs, and umbilical cord blood.

Main Results:

  • Achieved precise quantification of telomere length in kilobases per single copy gene copy number per chromosome.
  • Demonstrated high linearity and reproducibility across different sample types (blood, buccal swabs, cord blood).
  • The improved qPCR method proved to be rapid, cost-effective, and accurate.

Conclusions:

  • The enhanced qPCR technique provides a robust tool for absolute telomere length measurement.
  • This method supports large-scale telomere biology studies and improves clinical diagnostics.
  • Facilitates deeper understanding of telomere's role in aging and disease.