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Updated: May 3, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

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A Protocol for Regulating Protein Liquid-Liquid Phase Separation Using NMR-Guided Mutagenesis

Mayu Enomoto-Kusano1, Kyoko Furuita2,3, Takashi S Kodama2

  • 1Graduate School of Engineering Science, Yokohama National University, Tokiwadai 79-5, Hodogaya-ku, Yokohama 240-8501, Kanagawa, Japan.

Methods and Protocols
|February 20, 2026
PubMed
Abstract

Keywords:
NMR-guided mutagenesisVAPBliquid–liquid phase separationnuclear magnetic resonanceprotein condensatesprotein dynamics

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