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Postharvest application of plasma-activated water to extend shelf life of strawberries
Yingman Gao1, Yupan Zhu1, Panfeng Guan1
1Zhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450051, China; Henan Key Laboratory of Ion-beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, China; Sanya Institute, Zhengzhou University, Zhengzhou 450001, China.
Abstract:
Strawberries are vulnerable to postharvest microbial contamination, with gray mold from Botrytis cinerea being significant. Plasma-activated water (PAW) is a promising sustainable food sterilization method. This study evaluated PAW's efficacy in inhibiting postharvest strawberry microbial infections, delaying gray mold, and assessing quality, exploring its potential as an alternative to fungicides. Results showed PAW inhibited B. cinerea growth and delayed decay during storage. PAW reduced pathogenic-related genes in B. cinerea (BCBCG1, BCATG1, BCSTR2, BCSOD1) while enhancing defense-related genes in strawberries (FaPAL, FaPOD, FaCHI, FaGLU). To counteract PAW-induced reactive oxygen species (ROS), antioxidant-related genes (FaSOD, FaCAT, FaAPX, FaGR) are upregulated with increased enzyme activities. PAW treatment was associated with enhanced defense responses and redox homeostasis in strawberry fruit, potentially contributing to increased resistance to B. cinerea infection. In addition, PAW helped maintain strawberry quality during storage, thereby extending its shelf life.
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