Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complement System01:27

Complement System

11.2K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
11.2K
Renal Corpuscle01:20

Renal Corpuscle

7.8K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
7.8K
Proteomics01:33

Proteomics

9.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K
Nephrotic Syndrome II : Assessment and Medical Management01:26

Nephrotic Syndrome II : Assessment and Medical Management

265
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document...
265
Nephrotic Syndrome I : Introduction01:24

Nephrotic Syndrome I : Introduction

721
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
721

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GLP-1 Agonists and Risk of Atrial Fibrillation in Non-Dialysis Chronic Kidney Disease.

Cardiovascular drugs and therapy·2026
Same author

Evaluation of lighted catheter phototherapy in endoscopic treatment of urethral strictures: a rabbit pilot study.

International urology and nephrology·2026
Same author

Multiplexed Detection of Membranous Nephropathy Antigens by Multi-Reaction Monitoring Mass Spectrometry.

Kidney international reports·2026
Same author

The Kidneys Went Nuts: Oxalate Nephropathy From Excessive Cashew Nut Consumption.

Kidney medicine·2026
Same author

Serine protease HTRA1 is a common autoantigen in membranous nephropathy in patients of 80 years and older.

Kidney international·2026
Same author

From Clot Prevention to Kidney Injury: Revisiting Anticoagulant-Related Nephropathy.

Kidney & blood pressure research·2026
Same journal

Changes in Three-Dimensional Intrahepatic Biliary Structures in Patients With Hepatobiliary Diseases Visualized Using Tissue-Clearing Methods.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Genome-wide SNP-based Profiling of Loss of Heterozygosity Reveals Distinct Molecular Subgroup-specific Patterns in Gastrointestinal Stromal Tumors (GIST).

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

AI-Assisted HER2 Scoring in Breast Cancer: Diagnostic Agreement and Understanding Discordance.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Corrigendum to "POU2F3 in Small Cell Lung Cancer (SCLC): Diagnostic Utility in Neuroendocrine-Low/Negative SCLC and Discrimination From Other Thoracic Malignancies and Other Small Blue Round Cell Tumors" [Laboratory Investigation 2026;106(6):106124].

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Assessing the Effects of a 3D Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Transcription Factor Ets-1 Is a Central Regulator of Redox Balance and Liver Regeneration Through epidermal growth factor (EGF) and transforming growth factor-β (TGF-β) 1 Signaling.

Laboratory investigation; a journal of technical methods and pathology·2026
See all related articles

Related Experiment Video

Updated: Feb 22, 2026

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

3.6K

Proteomic Profiling of Complement Components in Glomerular Disease.

Aaron Storey1, Tiffany Caza1, Samar Hassen1

  • 1Arkana Laboratories, Little Rock, Arkansas.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|February 20, 2026
PubMed
Summary
This summary is machine-generated.

Mass spectrometry reliably measures complement proteins in kidney biopsies, offering a comprehensive view beyond standard tests. This advances understanding of complement

Keywords:
complementglomerular diseasekidney biopsymass spectrometry

More Related Videos

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

18.7K
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

751

Related Experiment Videos

Last Updated: Feb 22, 2026

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

3.6K
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

18.7K
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

751

Area of Science:

  • Nephrology
  • Immunology
  • Proteomics

Background:

  • The complement system is crucial in glomerular diseases.
  • Current biopsy methods (immunofluorescence) offer limited insight into complement activation.
  • Over 50 proteins are involved in the complement cascade, necessitating advanced detection methods.

Purpose of the Study:

  • To evaluate complement components comprehensively in kidney biopsies using mass spectrometry.
  • To explore the role of complement in various glomerular diseases.
  • To correlate complement protein abundance with disease activity and patient heterogeneity.

Main Methods:

  • Data-independent acquisition mass spectrometry (MS) was employed.
  • Proteins were extracted from kidney biopsy tissue (lysates, glomeruli, immunoprecipitates).
  • Cohorts included lupus nephritis, membranous nephropathy, diabetic glomerulosclerosis, C3 glomerulonephritis, and controls.

Main Results:

  • MS-based complement abundances correlated with C1q immunofluorescence.
  • Increased complement pathway proteins linked to lupus nephritis activity.
  • Complement component abundance showed heterogeneity across disease states, including diabetic glomerulosclerosis and proliferative glomerulonephritis.

Conclusions:

  • Mass spectrometry reliably quantifies complement proteins and activation products in kidney biopsies.
  • This approach enhances understanding of complement-mediated tissue injury and disease heterogeneity.
  • Multiplex detection of complement components offers a more detailed view of glomerular disease mechanisms.