Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ceramide-induced endoplasmic reticulum stress reveals a targetable vulnerability in endocrine therapy-resistant breast cancer.

Molecular cancer research : MCR·2026
Same author

Inhibition of Acid Sphingomyelinase Links Sphingolipid Remodeling to Necroptotic Cell Death.

bioRxiv : the preprint server for biology·2026
Same author

ER-localized ceramide accumulation contributes to replicative senescence.

Cell chemical biology·2026
Same author

Chronic PFOA and PFOS exposure triggers cellular oxidative stress and alters lipid levels as revealed through multi-omics analysis.

Environmental science. Advances·2026
Same author

Bioaccumulation of PFOS Isomers in Transporter Proteins.

Chemical research in toxicology·2026
Same author

Ceramide-induced Endoplasmic Reticulum Stress as a Targetable Vulnerability in Endocrine Therapy-Resistant Breast Cancer.

bioRxiv : the preprint server for biology·2025
Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026
Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026
Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026
Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026
Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026
Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026
See all related articles

Related Experiment Video

Updated: Feb 22, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
08:53

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

10.3K

High-resolution sphingolipid analysis in vitro using LC-qToF MS.

Shweta Chitkara1, G Ekin Atilla-Gokcumen1

  • 1Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY, United States.

Methods in Enzymology
|February 20, 2026
PubMed
Summary
This summary is machine-generated.

Advanced liquid chromatography-mass spectrometry (LC-MS) offers high sensitivity and precision for analyzing complex sphingolipids. This method enhances the understanding of cellular sphingolipid metabolism and its role in diseases.

Keywords:
CeramidesLC-MSLC-qToF MSSphingolipids

More Related Videos

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

7.9K
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
09:09

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

2.7K

Related Experiment Videos

Last Updated: Feb 22, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
08:53

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

10.3K
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

7.9K
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
09:09

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

2.7K

Area of Science:

  • Lipidomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • The sphingolipidome is complex, with variations in chain length, saturation, and headgroup composition.
  • Traditional biochemical methods struggle to differentiate closely related sphingolipid species.
  • Liquid chromatography-mass spectrometry (LC-MS) is crucial for high-sensitivity and structurally precise sphingolipid analysis.

Purpose of the Study:

  • To present an optimized liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-qToF MS) method for sphingolipid profiling.
  • To leverage LC-MS capabilities for accurate qualitative and quantitative analysis of sphingolipids.
  • To provide a foundation for dissecting sphingolipid metabolism and its dysregulation in cellular processes.

Main Methods:

  • Utilizing liquid chromatography-mass spectrometry (LC-MS) for separation and detection.
  • Employing liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-qToF MS) for high-resolution analysis.
  • Focusing on an optimized method for sphingolipid profiling in cultured mammalian cells.

Main Results:

  • LC-qToF MS provides high mass accuracy, a broad dynamic range, and rapid acquisition rates.
  • The method enables confident identification of isobaric and structurally related sphingolipids.
  • The approach offers a robust foundation for dissecting sphingolipid metabolism.

Conclusions:

  • LC-MS, particularly LC-qToF MS, is a powerful platform for sphingolipidomics.
  • The optimized method facilitates detailed analysis of sphingolipid profiles in mammalian cells.
  • This analytical strategy is essential for understanding sphingolipid roles in cellular physiology and pathology.