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Updated: Feb 22, 2026
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
Published on: October 15, 2016
High-resolution phosphoinositide analysis
Helene Jahn1, Berit Blume1, Alexis E Traynor-Kaplan2
1Oregon Health and Science University, Portland, OR, United States.
None:
Cells derived from diseased tissue and their related cell lines exhibit numerous metabolic changes, including variations in lipid composition and metabolism. Indeed, lipids are important biomarkers of various diseases and exhibit crucial signaling roles during disease states. However, lipids, especially low-abundant and transient lipids like phosphoinositides, are difficult to study due to a lack of sophisticated tools. Here, we describe a unique targeted lipidomics method that allows us to define and compare the phosphoinositide composition of diseased and healthy tissues as well as related cell lines.
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