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Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: Feb 22, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

High-resolution phosphoinositide analysis.

Helene Jahn¹, Berit Blume¹, Alexis E Traynor-Kaplan²

¹Oregon Health and Science University, Portland, OR, United States.

Methods in Enzymology

|February 20, 2026

Summary

This summary is machine-generated.

Researchers developed a new targeted lipidomics method to study phosphoinositides, crucial lipids in disease. This technique enables detailed comparison of lipid profiles in diseased versus healthy tissues and cell lines.

Keywords:

ESI-MS/MS Lipid analysis Lipidomics Phosphatidylinositol Phosphatidylinositol phosphates Phosphatidylinositol trisphosphate Phosphoinositides Targeted mass spectrometry

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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

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Last Updated: Feb 22, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

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Published on: January 6, 2016

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Area of Science:

Biochemistry
Cell Biology
Metabolomics

Background:

Cellular metabolism undergoes significant alterations in disease states, particularly concerning lipid composition and pathways.
Lipids serve as critical biomarkers for various diseases and play essential roles in disease-related signaling.
Studying low-abundance and transient lipids, such as phosphoinositides, is challenging due to limitations in current analytical tools.

Purpose of the Study:

To introduce and validate a novel targeted lipidomics methodology.
To enable precise definition and comparison of phosphoinositide profiles.
To investigate differences in phosphoinositide composition between diseased and healthy biological samples.

Main Methods:

Development of a unique targeted lipidomics approach.
Application of the method to analyze phosphoinositide content.
Comparative analysis of lipidomics data from diseased and healthy tissues and cell lines.

Main Results:

The described method successfully quantifies and compares phosphoinositide composition.
Distinct phosphoinositide profiles were identified between diseased and healthy samples.
The technique proved effective for studying low-abundant and transient lipid species.

Conclusions:

The novel targeted lipidomics method provides a powerful tool for phosphoinositide analysis.
This approach facilitates a deeper understanding of lipid alterations in disease.
The findings open new avenues for biomarker discovery and therapeutic strategies targeting lipid metabolism.