Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Eukaryotic Compartmentalizations01:46

Eukaryotic Compartmentalizations

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
Eukaryotic Compartmentalization01:46

Eukaryotic Compartmentalization

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Eukaryotic Compartmentalization01:37

Eukaryotic Compartmentalization

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
Entropy within the Cell01:22

Entropy within the Cell

A living cell's primary tasks of obtaining, transforming, and using energy to do work may seem simple. However, the second law of thermodynamics explains why these tasks are harder than they appear. None of the energy transfers in the universe are completely efficient. In every energy transfer, some amount of energy is lost in a form that is unusable. In most cases, this form is heat energy. Thermodynamically, heat energy is defined as the energy transferred from one system to another that is...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aerobic oxidative condensation for the synthesis of phenoxazinone and phenazine derivatives catalyzed by an iron-porphyrin complex with a pendant imidazole ligand.

RSC advances·2026
Same author

Psychological profiles of mindfulness and tourist fatigue: a latent profile analysis in a digital travel context.

Scientific reports·2026
Same author

Investigating the crystalline structure and structural heterogeneity of starch granules using polarization-based quantitative phase microscopy.

Food chemistry·2026
Same author

Micropeptide YG-6 encoded by exosomal LINC01123 derived from highly migratory ovarian cancer cells promotes tumor progression.

Molecular cancer·2026
Same author

Spacer cations: molecular switches for 2D and 2D/3D perovskite photovoltaics.

Nanoscale·2026
Same author

MFST-GCN: A Sleep Stage Classification Method Based on Multi-Feature Spatio-Temporal Graph Convolutional Network.

Brain sciences·2026
Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026
Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026
Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026
Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026
Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026
Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: May 11, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.5K

Spatial perturb-seq: single-cell functional genomics within intact tissue architecture.

Kimberle Shen1, Wan Yi Seow1, Choong Tat Keng1

  • 1Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR), 60 Biopolis Street, Genome, Singapore, Republic of Singapore.

Nature Communications
|February 21, 2026
PubMed
Summary
This summary is machine-generated.

Spatial Perturb-Seq enables in vivo CRISPR screening of multiple genes in intact tissues. This method reveals cell-specific and microenvironmental impacts of gene knockouts, aiding neurodegenerative disease research.

More Related Videos

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

631
Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

856

Related Experiment Videos

Last Updated: May 11, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.5K
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

631
Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

856

Area of Science:

  • Genomics
  • Neuroscience
  • Molecular Biology

Background:

  • Understanding gene function in complex tissues is crucial for disease research.
  • Existing methods often struggle to maintain spatial context and cell representation during analysis.
  • Neurodegenerative diseases involve complex genetic and environmental interactions within the brain.

Purpose of the Study:

  • To develop a novel in vivo CRISPR technology for high-throughput functional genetic screening in intact tissues.
  • To investigate the cell-autonomous and cell-cell microenvironmental effects of gene knockouts relevant to neurodegenerative diseases.
  • To identify candidate genes involved in neuronal intercellular communication.

Main Methods:

  • Spatial Perturb-Seq: an in vivo CRISPR technology for interrogating multiple genes within single cells in intact tissues.
  • Compatibility with both sequencing-based and probe-based spatial technologies.
  • Application to knockout risk genes for neurodegenerative diseases in the mouse brain.

Main Results:

  • Successfully applied Spatial Perturb-Seq to functionally screen multiple genes in situ and in vivo.
  • Bypassed cell processing steps that can skew cell type representation, preserving tissue architecture.
  • Identified both intracellular and intercellular effects of gene knockouts.
  • Uncovered cell autonomous and cell-cell microenvironmental effects within the spatially intact mouse brain.
  • Identified candidate genes underlying dysregulated neuronal intercellular communication pathways.

Conclusions:

  • Spatial Perturb-Seq is a powerful tool for in vivo functional genomics in intact tissues.
  • The technology elucidates complex gene interactions and microenvironmental influences in neurodegenerative disease models.
  • This approach advances the study of neuronal communication and identifies potential therapeutic targets.