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Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds
Published on: August 21, 2021
Formulation and evaluation of hydrogel based on Brazilian propolis ethanol extract for promoting diabetic wound
Huanglin Huo1, Zhenhua Zhou1, Rongxiang Guo2
1The First Affiliated Hospital of Guangdong Pharmaceutical University, MOE Key Laboratory of Glucolipid Metabolic Disorder, Guangdong Provincial TCM Key Laboratory for Metabolic Diseases, Traditional Chinese Medicine Research Institute (Guangdong Provincial Research Center of Integration of Traditional Chinese Medicine and Western Medicine in Metabolic Diseases), Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Abstract:
Diabetes wounds represent a significant clinical challenge, primarily attributed to their complex microenvironment characterized by persistent inflammation, heightened infection susceptibility, and impaired angiogenesis. To address these interconnected challenges, a multifunctional hydrogel dressing (designated EEP/PS) was constructed using pullulan, Brazilian propolis ethanolic extract, and polyvinyl alcohol. This biomaterial exhibits an interconnected porous structure, suitable mechanical properties, tissue adhesion, remarkable thermal stability, excellent sustained-release and swelling capacity. Notably, EEP/PS shows potent antioxidant capability and significantly enhances cell migration in hydrogen peroxide-induced oxidative injury models. Furthermore, the hydrogel downregulates the expression and secretion of key pro-inflammatory cytokines in LPS-stimulated macrophages, including tumor necrosis factor-α, interleukin-6, and interleu-kin-1β. The hydrogel also exhibits broad-spectrum antibacterial efficacy against common wound pathogens Escherichia coli and Staphylococcus aureus. In a diabetic rat model, EEP/PS accelerated wound closure by synergistically promoting reepithelialization, collagen deposition, angiogenesis while mitigating local inflammation. Additionally, EEP/PS exhibits an excellent hemostasis capacity and bio-compatibility, critical for clinical translation. Collectively, these findings highlight the translational potential of EEP/PS as a multifunctional biomaterial for diabetic wound care.
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