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Updated: Feb 24, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
Published on: April 23, 2014
Derivation of an iPSC line using the Sendai virus from a newborn boy with Alagille Syndrome
Rui Zhou1, Pan Liu2, Xiaokang Peng2
1Department of Infectious Diseases, Xi'an Children's Hospital, Xi'an, China; National Regional Children's Medical Center (Northwest), Key Laboratory of Precision Medicine in Pediatric Diseases of Shaanxi Province, Xi'an Key Laboratory of Children's Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi'an Children's Hospital, Affiliated Children's Hospital of Xi'an Jiaotong University, Xi'an, China.
Abstract:
We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.
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