Related Experiment Video
Updated: Feb 24, 2026
Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
Published on: January 19, 2019
Establishment single lung tumors through orthotopic injection
Beñat Picabea1, Céline Clémenson2, Mirari Echepare3
1Program in Solid Tumors, Cima-University of Navarra, Pamplona, Spain; Department of Biochemistry and Genetics, School of Sciences, University of Navarra, Pamplona, Spain; Consorcio de Investigación Biomédica en Red, Cáncer (CIBERONC), Madrid, Spain.
This study introduces an orthotopic lung cancer model for more accurate preclinical research. This advanced model better reflects human lung cancer progression and microenvironment interactions for improved therapeutic development.
Area of Science:
- Oncology
- Preclinical Research
- Animal Models
Background:
- Lung cancer is a leading cause of cancer mortality, often diagnosed late.
- Subcutaneous murine models inadequately represent the human tumor microenvironment.
- Understanding the tumor microenvironment is critical for non-small cell lung cancer (NSCLC) therapies.
Purpose of the Study:
- To present a protocol for orthotopic tumor implantation in mice.
- To highlight the advantages of orthotopic models over subcutaneous models for lung cancer research.
- To emphasize the importance of accurate models for developing diagnostic and therapeutic strategies.
Main Methods:
- Orthotopic implantation of lung cancer cells in mice.
- Tumor volume measurement using Luciferase Bioluminescence (BLI) or Computerized Tomography (CT).
Main Results:
- Orthotopic models more accurately mirror human lung cancer progression and metastasis.
- These models better reflect tumor microenvironment interactions.
- Orthotopic models provide a more realistic platform for evaluating treatment efficacy.
Conclusions:
- Orthotopic lung cancer models are superior to subcutaneous models for preclinical research.
- Accurate tumor microenvironment simulation is essential for advancing lung cancer therapies.
- This protocol facilitates the development of improved diagnostic and therapeutic strategies for lung cancer.
