Hidden hosts: Limpets as key players in trematode transmission

Limpets are abundant and ecologically important gastropods in intertidal and some riverine ecosystems, yet their role in trematode transmission remains comparatively understudied. We investigated trematode infection in intertidal limpets from Otago Harbour, New Zealand, using a molecular approach. Two species were identified: the avian schistosome Gigantobilharzia cf. patagonensis in Siphonaria australis and Acanthoparyphium sp. A metacercariae in Notoacmea sp. Gigantobilharzia cf. patagonensis was detected at all five sampling sites, with prevalence ranging from 2.6% to 100%. Haplotype network analysis using cox1 revealed high haplotype diversity and a star-like topology, suggesting a recent population expansion. This study expanded the known diversity of marine schistosomes in the region and raises potential public health concerns with cercarial dermatitis. We also conducted a literature synthesis further demonstrating that limpets contribute to trematode transmission across 12 superfamilies and 23 families, with distinct parasitism profiles between freshwater and marine environments. These findings highlight limpets as overlooked but significant hosts in trematode ecology and emphasize the need for broader surveys and definitive host screening to resolve incomplete life cycles and assess epidemiological risks in coastal ecosystems.