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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

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Related Experiment Video

Updated: Feb 24, 2026

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

Genome-wide single-cell perturbation screens with VIPerturb-seq.

Alexandra Bradu^1,2, John D Blair^1,2, Isabella N Grabski^1,2

¹New York Genome Center, New York, NY.

Biorxiv : the Preprint Server for Biology

|February 23, 2026

Summary

This summary is machine-generated.

VIPerturb-seq enhances CRISPR screening with single-cell sequencing for genome-wide genetic perturbation mapping. This scalable platform improves throughput and efficiency for molecular phenotype analysis.

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Last Updated: Feb 24, 2026

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Published on: August 25, 2023

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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

CRISPR screening coupled with single-cell sequencing (Perturb-seq) maps genetic perturbations to molecular phenotypes.
Current Perturb-seq methods face cost and throughput limitations for genome-wide screens.

Purpose of the Study:

Introduce VIPerturb-seq, a platform for routine, cost-effective, genome-wide Perturb-seq.
Enhance scalability and efficiency of large-scale genetic screens.

Main Methods:

Developed a split probe strategy for detecting genome-wide CRISPR libraries in fixed cells.
Integrated optional phenotypic enrichment for Very Important Perturbations (VIP).
Enabled combinatorial indexing for a 50-fold increase in Perturb-seq throughput.

Main Results:

Demonstrated VIPerturb-seq using the genome-wide CRISPRi library (GuEST-List).
Successfully conducted both unbiased and phenotypically enriched genome-wide screens.
Validated the sensitivity, scalability, and efficiency of the VIPerturb-seq platform.

Conclusions:

VIPerturb-seq facilitates routine genome-wide Perturb-seq experiments.
The platform supports both targeted research and large-scale data generation for virtual cell construction.
VIPerturb-seq significantly advances the capacity for comprehensive genetic perturbation analysis.