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Updated: Feb 24, 2026

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

A pilot study for whole proteome tagging in C. elegans.

Matthew Eroglu¹, Oliver Hobert¹

¹Columbia University, Department of Biological Sciences, Howard Hughes Medical Institute, New York, NY 10027.

Biorxiv : the Preprint Server for Biology

|February 23, 2026

Summary

This pilot study efficiently tagged 30 essential genes in C. elegans using three distinct fluorescent proteins. The approach revealed novel patterns in protein expression and localization, paving the way for whole-genome tagging.

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Area of Science:

Genomics
Molecular Biology
Biochemistry

Background:

Fluorescent tagging of all proteins in an animal genome can reveal novel expression and localization patterns.
Scaling this approach requires parallel introduction of multiple, spectrally distinct fluorophore tags.

Purpose of the Study:

To perform a pilot study in C. elegans to tag 30 genetic loci with three different fluorophores simultaneously.
To explore challenges related to gene function disruption and tagged protein visibility.

Main Methods:

Selected 30 essential genes in C. elegans based on transcriptomics for varied expression levels.
Introduced three spectrally distinct fluorophore tags in parallel at these genetic loci.

Main Results:

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Last Updated: Feb 24, 2026

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Published on: May 23, 2020

Demonstrated high efficiency of the simultaneous multi-fluorophore tagging approach.
Uncovered previously unknown cellular sites of expression and subcellular protein localization patterns.

Conclusions:

This pilot study validates the efficiency and utility of parallel multi-fluorophore tagging.
Motivates scaling this technique for comprehensive whole-genome protein analysis.