Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low-Dose H<sub>2</sub>O<sub>2</sub> Priming Improves Performance Under Simulated Marine Heatwave Conditions in a Coastal Bivalve.

Global challenges (Hoboken, NJ)·2026
Same author

Nanohybridization as a Route to a Water-Friendly Therapeutic Tool for Rescuing Misfolded Proteins.

ACS nanoscience Au·2026
Same author

Predicting NMR Relaxation Using a First-Principles Brownian Dynamics Approach.

Journal of chemical theory and computation·2026
Same author

Antimicrobial and Anti-Inflammatory Bioactive Peptides: Their Role in Potential Therapeutic Applications for Periodontitis-A Narrative Review.

Nutrients·2025
Same author

An Integrated Approach in Assessing the Food-Related Properties of Microparticulated and Fermented Whey.

Foods (Basel, Switzerland)·2025
Same author

Molecular dynamics of space-confined water inside Span 80 reverse micelles with an all-atom and coarse-grained solvent.

Physical chemistry chemical physics : PCCP·2025
Same journal

Efficient and Stable Ligand-Exchange Process Using Perovskite Nanocrystals Synthesized from a Liquid Crystalline Antisolvent.

ACS nanoscience Au·2026
Same journal

Activation of the Complement System: Morphological Differences in the Surface of PIBCA Nanoparticles Coated with Polysaccharides.

ACS nanoscience Au·2026
Same journal

Structural Stability of Sulfur-Depleted MoS<sub>2</sub>.

ACS nanoscience Au·2026
Same journal

Structural Complexity from Consecutive Postsynthetic Transformations: The Site-Specificity of Sequential Tellurium Anion and Cadmium Cation Exchange on Roxbyite Copper Sulfide Nanoparticles.

ACS nanoscience Au·2026
Same journal

Accurate Prediction of Optical Transitions in Epitaxial InGaAs/InAlAs Asymmetric Coupled Quantum-Well Structures.

ACS nanoscience Au·2026
Same journal

Transport Evidence of Surface States in Magnetic Topological Insulator MnBi<sub>2</sub>Te<sub>4</sub>.

ACS nanoscience Au·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
09:29

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

12.7K

Plasmonic Nano-bolas Hunt DNA Targets.

Aura Cencini1, Graziano Rilievo1, Mirco Zerbetto2

  • 1Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università 16, 35020, Legnaro (PD), Italy.

ACS Nanoscience Au
|February 23, 2026
PubMed
Summary
This summary is machine-generated.

We developed a DNA-scaffolded nanomachine using nano-bolas for ultrasensitive detection. This reversible plasmonic reporter system offers improved signal-to-noise ratio and kinetics for nucleic acid analysis.

Keywords:
DNA nanotechnologyfluorescencegold nanoparticlequenching-enhancingself-assembly

More Related Videos

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

4.1K
Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
09:33

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

1.5K

Related Experiment Videos

Last Updated: May 7, 2026

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
09:29

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

12.7K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

4.1K
Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
09:33

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

1.5K

Area of Science:

  • Nanotechnology
  • Biochemistry
  • Molecular Engineering

Background:

  • DNA nanotechnology enables the construction of complex molecular machines.
  • Controlling nanoparticle proximity is crucial for developing nanoscale sensors.

Purpose of the Study:

  • To design and assemble a reconfigurable DNA-scaffolded nanomachine.
  • To compare single-molecule (nano-bolas) and bi-molecule DNA scaffolds for optimal performance.
  • To evaluate the nanomachine as a plasmonic reporter for nucleic acid detection.

Main Methods:

  • DNA strand displacement mechanism to operate the nanomachine.
  • Utilizing a fluorophore and two gold nanoparticles (AuNPs) on a DNA scaffold.
  • Comparing single-molecule nano-bolas with bi-molecule scaffolds for reversibility, response, and signal-to-noise ratio.

Main Results:

  • Single-molecule nano-bolas exhibited superior reversibility, lower noise, and better kinetics compared to bi-molecule scaffolds.
  • The nanomachine demonstrated discrete emission levels based on system state and AuNP proximity.
  • Observed distance-dependent responses and kinetics aligned with theoretical modeling.

Conclusions:

  • Nano-bolas are optimal scaffolds for DNA-nanomachines due to minimal cross-talk and high reversibility.
  • The developed nanomachine functions as an ultrasensitive, reversible plasmonic reporter.
  • Potential applications include the detection of single-strand nucleic acid analytes.