Experimental Study on the Influence of Different Storage Conditions (Temperature, Duration) on Platelet Count, LDH,...

Currently, platelet-rich plasma (PRP) is stored at -80 °C, requiring deep cryogenic freezers not widely available in hospital. More research is required to optimize clinical PRP preservation conditions. PRP from Deyang City People's Blood Center were stored at -20 °C, -40 °C, and - 80 °C. Platelet counts, LDH concentration, and growth factors were measured at 0, 45, 90, and 180 days using a hematology analyzer and ELISA. Analysis was conducted using GraphPad Prism 9 to compare differences between storage temperatures and over time within each group. During 180 days of PRP storage, platelet counts declined by 97.95%, 9.99%, and 7.51% at -20 °C, -40 °C, and - 80 °C, respectively, compared to day 0.LDH levels peaked before declining. Growth factors showed diverse patterns: EGF rose steadily, PDGF-AA and TGF-βinitially increased, then decreased, while PDGF-BB and VEGF fluctuated, and FGF2 decreased consistently. At 45 days, PDGF-BB and PDGF-AA were significantly higher, and TGF-βlower in the - 20 °C group compared to -80 °C (P < 0.05). No significant differences were found in EGF, FGF2, PDGF-AA, and VEGF levels between - 40 °C and - 80 °C storage (P > 0.05). Storage at -40 °C showed comparable outcomes to -80 °C, indicating its suitability for PRP storage. While - 20 °C storage initially boosts growth factors, a significant platelet decline within 45 days cautions against long-term clinical use. Limiting PRP storage at -20 °C to 45 days ensures quality.