Related Experiment Video
Updated: Feb 24, 2026
06:35
Cell Membrane Repair Assay Using a Two-photon Laser Microscope
Published on: January 2, 2018
13.5K
The LDL pathway regulates actomyosin ring dynamics necessary for optimal cell wound repair
Geethika Burugupally1, Mitsutoshi Nakamura1, Cassandra Aarrestad1
1Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.
Micropublication Biology
|February 23, 2026
Summary
The Low-Density Lipoprotein (LDL) pathway is crucial for cell wound repair. This pathway unexpectedly regulates actin dynamics, ensuring robust actomyosin ring formation for effective cell repair.
Area of Science:
- Cell biology
- Molecular biology
- Wound healing research
Background:
- Cellular damage requires rapid repair for survival and tissue function.
- Actomyosin ring formation is essential for physically closing cell wounds.
- The Low-Density Lipoprotein (LDL) pathway typically maintains plasma membrane homeostasis.
Purpose of the Study:
- To investigate the role of the LDL pathway in cell wound repair.
- To understand how the LDL pathway influences actomyosin ring formation.
- To uncover novel functions of the LDL pathway in regulating cellular processes.
Main Methods:
- Cell culture models to induce and observe cortical damage.
- Live-cell imaging to monitor actomyosin ring dynamics.
- Biochemical assays to assess LDL pathway activity and actin regulation.
Main Results:
- The LDL pathway is essential for efficient cell wound repair.
- Impaired LDL pathway function hinders actomyosin ring formation.
- The LDL pathway plays an unexpected role in regulating actin dynamics during repair.
Conclusions:
- The LDL pathway is a key regulator of cell wound repair.
- This pathway is critical for robust actomyosin ring assembly.
- Discovering a novel function for the LDL pathway in actin dynamics during cellular repair.
More Related Videos
Related Concept Videos
01:28Phases of Wound Repair
9.0K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
9.0K
01:31Mechanism of Lamellipodia Formation
3.8K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.8K
02:15The Contractile Ring
7.4K
Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
7.4K
01:10The Role of Actin and Myosin in Non-muscle Cells
5.2K
Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They are held...
5.2K
01:16Cell Motility through Blebbing
2.6K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
2.6K
01:32Cytoskeletal Coordination in Cell Migration
5.6K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.6K