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A Small Molecule That Inhibits the Quorum Sensing Receptor AgrC in Staphylococcus aureus
Thomas J Polaske1, Troy D Vulpis1, Alexandra E Nelson1
1Department of Chemistry, University of Wisconsin-Madison, 1101 University Ave., Madison, Wisconsin 53706, United States.
Abstract:
We report the synthesis and characterization of a small molecule, CP-20, capable of inhibiting the key quorum sensing receptor AgrC in Staphylococcus aureus. Structural alterations enabled the discovery of a CP-20 derivative that is capable of complete quorum sensing inhibition with mid-nanomolar potency. In vitro biochemical investigations support CP-20 binding AgrC and competitively inhibiting its activity. CP-20 represents a versatile and synthetically tractable small molecule probe for investigating Staphylococcal virulence via quorum sensing modulation.
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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

